中文摘要：

背景：通过分子生物学技术与组织工程技术相结合以修复骨缺损，是骨科当前重要的研究方向之一。转化生长因子β1作为重要的骨形成因子，在骨代谢和骨损伤修复中发挥着极为重要的作用。 目的：观察转化生长因子βl基因修饰的成骨细胞复合仿生基质材料修复鼠胫骨骨缺损的治疗效果。 设计：随机对照实验。 单位：华中科技大学同济医学院附属协和医院骨科。 材料：实验于2003-03／08在华中科技大学同济医学院附属协和医院完成。选用健康SD大鼠20只，雌雄不限，由华中科技大学同济医学院实验动物中心提供。 方法：将转化生长因子βl基因转染的成骨细胞与涂覆多聚赖氮酸的聚DL乳酸仿生基质材料复合，植入鼠胫骨骨缺损模型，术后摄X射线片和组织学检查观察修复情况。 主要观察指标：术后4，8周X线摄片和组织学检查情况。 结果：20只SD大鼠均进如结果分析，无脱失。①实验组：4周时X线片可见骨痂形成，组织学观察有类骨组织和新骨形成，成骨细胞贴附于基质材料表面。8周时缺损基本修复，新骨密度与自体骨接近。②对照组：4周时X线片未见骨痂形成，组织学观察没有类骨组织形成，基质材料表面成骨细胞贴附较少，材料腔隙中有大量淋巴细胞浸润。8周时植入材料基本吸收，为纤维组织替代。 结论：将分子生物学和组织工程学结合修复骨缺损，获得理想的治疗效果。

英文摘要：

BACKGROUND： How to deal with bone defect is a big problem to surgeons. In recent years, the development in the technology of molecular biology and tissue engineering provides broad prospect for the clinical treatment of bone defect, which is one of the important study directions in department of orthopedics. The transforming growth factor-beta I（TGF-β1）, one of the important factors in bone formation, plays an important role in bone metabolism and recovery. OBJECTIVE： To observe the therapeutic effects of bone defects with osteoblasts＇ transfected by TGF-β1 combining with biomimetic biodegradable polymer scaffolds. DESIGN： Randomized controlled trial. SETTING： Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS： Siderophilin, trypsin, 3H-proline and sirius red, etc. MATERIALS： The experiment was completed in Union Hospital, Tongji Medical College, Huazhong University of Science and Technology from March to August in 2003. Twenty healthy adult Sprague-Dawley（SD） rats of SPF grade, weighing 200-250 g, were provided by the Experimental Animal Center of Tongji Medical College. METHODS： The osteoblasts transfected by TGF-β1 gene, combining with poly-DL-lactic acid scaffolds modified with poly-L-lysine, were transplanted into rat tibia defect. Radiographs and histological analysis were performed to evaluate the repair effects. MAIN OUTCOME MEASURES： The X-ray evaluation and histology observation were performed at the 4th and 8th weeks after the operation. RESULTS： Totally 20 SD rats were included in result analysis without one rat missing. ①ln the experiment group, X-ray image indicated callus formation, while histology observation showed osteoid tissue and new bone formation, and osteoblasts attached to the surface of the materials after 4 weeks. Eight weeks later, the defect was essentially repaired, and the bone density of new bone was similar to that of the autogenous bone. ②ln the control group, t