目的 应用微吸管吸吮技术测量骨髓基质细胞在聚L-乳酸(PLLA)、丙交酯/乙交酯(PLGA)、聚丙交酯/乙交酯/天冬氨酸/聚乙二醇(PLGA-EASPPEG])聚合物表面的黏附力,比较3种材料的细胞黏附性,以筛选生物相容性好的生物材料。方法 在微吸管吸吮装置下,测量不同时间段骨髓基质细胞在各种生物材料表面的黏附力。结果 细胞种植在生物材料表面4、12、24h,各时间段PLGA-[-ASP—PEG]表面的细胞黏附力均最强,PLGA表面的黏附力强于PLLA。结论 在PLLA、PLGA、PLGA-[AsP-PEG]中,PLGA-EASP—PEG]表面的细胞黏附力最好,是理想的生物材料。
Objective To examine the characteristics of marrow stromal cells adhesion to poly(L-lactide) acid (PLLA), poly(lactic co glycolic) acid (PLGA) and poly(lactic acid/glycolic acid/asparagic acid-copolyethylene glycol) (PLGA-EASP- PEG]). Methods The adhesion force of MSCs to different biomaterials in diferrent time points was detected by using a micropipette aspiration technique. Results At 4th, 12th and 24th h after MSCs inoculation in biomerials, the adhesion force of PLGA-[-ASP-PEG-] was strongest among the three polymers, followed by PLGA. Conclusion Characteristics of MSCs adhesion to PLGA-[ASP-PEG] is best among the three polymers. PLGA-[-ASP-PEG-] is an ideal scaffold for bone tissue engineering.