中文摘要：

目的：探讨补骨脂黄酮对体外培养的成骨细胞增殖和矿化成熟的影响。方法：取新生SD大鼠颅骨，体外培养成骨细胞，取第1代细胞进行以下实验。①将细胞分为6组，A组不进行干预，B、c、D、E、F组分别采用终浓度为1×10-1mol·L-1、l×10。mol·L-1、1×10-1mol·L、1X10mol·L、l×10mol·L。。的补骨脂黄酮进行干预，检测细胞增殖情况。，②将成骨诱导培养的细胞分为6组，a组不进行干预，b、c、d、e、f组分别采用终浓度为lX10μmol·L-1、1×10-1mol·L-1、1×10-1mol·L-1、1×10-1mol·L-1、1×10-mol·L“的补骨脂黄酮进行干预，9d后检测碱性磷酸酶活性，将碱性磷酸酶活性最高者确定为补骨脂黄酮的最佳浓度。③采用ELISA法检测a组和碱性磷酸酶活性最高组成骨诱导培养3d、6d、9d、12d和15d时培养液中骨钙素、骨形态发生蛋白-2、骨桥蛋白和I型胶原蛋白的含量。④成骨诱导第12天，采用茜素红染色法检测a组和碱性磷酸酶活性最高组钙化结节的形成情况。⑤采用PCR法测定a组和碱性磷酸酶活性最高组成骨培养开始后72h内碱性成纤维细胞生长因子mRNA、胰岛素样生长因子-1mRNA、转录因子Runx-2mRNA和OsterixmRNA的表达水平。结果：①细胞增殖测定结果？6组吸光度值比较，差异无统计学意义[（O．512±0．046），（0．448±0．051），（0．528±0．043），（0．525±0．041），（0．522±0．039），（0．517±0．049），F=1．438，P=0．282l。②碱性磷酸酶活性检测结果。6组吸光度值比较，差异有统计学意义[（2．637±0．221），（2．136±0．168），（3．678±0．235），（3．153±0．201），（3．001±0．224），（2．934±0．188），F=15．442，P=0．000l；a组吸光度值高于b组（P：0．018），低于其余4组（P=0．000，P=0．003，P=0．016，P=0．043），c组高于b、d、e、f组（P=0．000，P=0．034，P=0．013，P=0．001）。③骨相关蛋白?

英文摘要：

Objective：To explore the effect of bavachin on proliferation and maturation of osteoblast cuhured in vitro. Methods：The skulls were taken from SD neonatal rats for osteoblast culture, and the first-generation cells were chosen for the following experiments. The cells were divided into 6 groups,and cells in group A were not intervened,while cells in other groups were placed in the culture fluids re- spectively added with bavachin which final concentration were 1 x 10-4 mot/L（ group B）, 1 ~ 10 5 mot/L（ group C） , 1 x 10 6 mol/I, （group D） , 1 x 10-7 mol/L（ group E） , 1 x 10-8 mot/L（ group F）, and the cells proliferation were detected. The cells were divided into 6 groups after osteogenic induction, and cells in group a were not intervened, while cells in other groups were placed in tile culture tluids respectively added with bavachin which final concentration were 1 x 10-4 mol/L（ group b）, 1 x 10-5 mol/L（ group c）, 1 x 10-6 moL/L （group d） , 1 x 10-7 tool/L（ group e） , 1 x 10-8 tool/L（ group f）. The alkaline phosphatase（ALP） activities were detected 9 days later and the optimal concentration of bavachin was determined by the highest ALP activities. The content of osteoealcin （ OC）, bone morphogenetie proteins-2 （BMP-2）, osteopontin （OPN）and type- I collagen protein in the culture solution of group a and the group with the highest ALP activity were detected through ELISA 3,6,9,12 and 15 days after osteogenic induction respectively. The numbers of calcified nodules in group a and the group with the highest ALP activity were detected through alizarin red staining 12 days after osteogenic induction. The ex- pression level of basic fibroblast growth factor（bFGF） mRNA, insulin-like growth factor-1 （ IGF-1 ） mRNA, Runx-2 mRNA and Osterix mR- NA in group a and the group with the highest ALP activity were detected through PCR within 72 hours after the culture. Results：The cells proliferation detection showed there were no statistical differences in optica