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新伪尾柱虫腹皮层纤毛器微管胞器的形态和形态发生
  • 期刊名称:动物学杂志
  • 时间:0
  • 页码:74-80
  • 语言:中文
  • 分类:Q244[生物学—细胞生物学] Q959.117[生物学—动物学]
  • 作者机构:[1]School of Life Science, East China Normal University, Shanghai 200062, China
  • 相关基金:Supported by the National Natural Science Foundation of China (No 30770238)
  • 相关项目:原生动物纤毛虫的纤毛器微管及微管组织中心的研究
中文摘要:

我们检验了结构和 ciliature 的空间组织在三 hypotrichous ciliates 的联系底的微导管(欺骗)(Stylonychia mytilus, Pseudourostyla cristata, Euplotes woodruffi ) 在里面荧光显微镜学。结果揭示了那欺骗,包括前面(ALM ) ,以后的纵的微导管(PLM ) 和横向的微导管(TM ) 乐队,由导管素组成。各自的微导管乐队有显著地不对称的细胞质的极化模式。在 P 的 midventral 文件的欺骗。cristata 在两 S 与横向的触毛的 ALM 乐队相比显得塑造绳索。mytilus 和 E。woodruffi,它在收敛前延长到房间的左前面的方面。在 S 的左边缘的触毛(MC ) 的 TM 乐队。mytilus 延长到房间的右边,当那些正确 MC 乐队延长到左时。尽管,我们的观察建议欺骗特点在 hypotrichous ciliates 是普通的不同种类拥有与保存 cirral 有关的不同微导管安排在各自的种类的形态基因的模式。欺骗在的不同发展三有睫建议微导管可以在不同 hypotrichs 被保存。我们也证明了 BAM,显得极、不对称,在特定的细胞骨架位置是局部性的并且在外皮以内在不同取向延长与另外的联系 ciliature 的结构连接并且因此,加强外皮。这些欺骗特征显示他们直接与房间运动被联系。关键词微导管 - 腹的外皮 - hypotrichous 有睫 - 导管素由中国(号码 30770238 ) 的国家自然科学基础支持了

英文摘要:

We examined the structure and spatial organization of ciliature base-associated microtubules (BAM) in three hypotrichous ciliates (Stylonychia mytilus, Pseudourostyla cristata, Euplotes woodruffi) in fluorescence microscopy. The results revealed that BAM, including the anterior (ALM), posterior longitudinal microtubule (PLM) and the transverse microtubule (TM) bands, are composed of tubulin. The respective microtubular bands have cytoplasmic polarization patterns that are significantly asymmetric. The BAM of the midventral files in P. cristata appear cord-shaped compared with the ALM bands of transverse cirri in both S. mytilus and E. woodruffi, which extend to the left anterior side of the cell before converging. The TM bands of the left marginal cirri (MC) in S. rnytilus extend to the right side of the cell, while those of the right MC bands extend to the left. Our observations suggest that BAM traits are common in hypotrichous ciliates even though different species possess different microtubule arrangements related to the conserved cirral morphogenetic patterns in the respective species. The differing development of BAM in the three ciliate suggests that the microtubules may be conserved in different hypotrichs. We have also demonstrated that the BAM, which appear polar and asymmetric, are localized in specific cytoskeletal positions and extend in different orientations within the cortex to connect with other ciliature-associated structures and, thus, strengthen the cortex. These BAM features indicate that they are directly associated with cell motion.

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