通过对蛋白核小球藻进行细胞密度的测定,分析了不同浓度的柳树(Salix babylonica)叶浸提液对蛋白核小球藻(Chlorella pyrenoidosa)生长和细胞形态的影响。结果表明:柳树叶浸提液对蛋白核小球藻具有化感作用,不同浓度的浸提液化感作用强度不同;作用96 h的半效应浓度(EC50,96 h值)为11.82 g·L^-1,较低浓度(10和20 g·L^-1)浸提液对蛋白核小球藻表现出先抑制后促进的作用特性,而较高浓度(30 g·L^-1)浸提液则始终表现出抑制效果;10、20和30 g·L^-1浸提液对蛋白核小球藻的最大抑制率分别为37.5%、39.3%和56.5%;柳树叶浸提液作用下,部分蛋白核小球藻细胞平均颗粒体积增大,颜色变透明,膨胀后溶解;低浓度浸提液处理组少数细胞聚集在一起,高浓度浸提液处理组细胞大量聚集,形成多细胞群体,多数沉于瓶底,沉降性能增强。
The allelopathic effects of Salix babylonica leaf extracts on the growth and morphology of Chlorella pyrenoidosa were investigated by measuring the cell density of treated algae. It was shown that the leaf extracts of S. babylonica had definite allelopathic effects on C. pyrenoidosa, depending on their concentration. The EC50. 96 h value of the extracts was 11.82 g · L^- 1. Lower concentrations (10 and 20 g · L^-l) of the extracts inhibited the algal growth first and promoted it then, while higher concentration (30 g · L^-1 ) of the extracts showed inhibitory effect all the time. The maximum inhibitory rate of the extracts at concentrations of 10, 20, and 30 g · L^-1 was 37.5%, 39.3%, and 56.5%, respectively. After treated with the extracts, part of C. pyrenoidosa ceils aggregated, expanded, turned transparent, and finally lysed. The amount of the aggregated cells increased with increasing concentration of the extracts. When treated with 30 g · L^-1 of the extracts, C. pyrenoiclosa cells largely aggregated, and precipitated as cell pellets to the bottom of the container.