中文摘要：

目的：研究组蛋白HIST1H2AC和HIST1H2BC在培美曲塞诱导的胰腺癌耐药细胞株Puta8988中的基因和蛋白表达水平，以明确HIST1H2AC和HIST1H2BC基因与胰腺癌耐药的关系。方法：以两种剂量的培美曲塞诱导胰腺癌细胞株Puta8988，使其形成两个等级的获得性耐药细胞株Puta8988＋、Puta8988＋＋。采用qRT-PCR和Western blotting方法分别检测HIST1H2AC和HIST1H2BC在耐药细胞株及其亲本（对照）中的基因和蛋白表达水平。结果：qRT-PCR检测结果显示，组蛋白HIST1H2AC和HIST1H2BC基因在胰腺癌耐药株Puta8988＋和Puta8988＋＋中的表达水平显著上调（P〈0．05）；Western blotting检测结果显示，组蛋白HIST1H2AC和HIST1H2BC在胰腺癌耐药株Puta8988＋和Puta8988＋＋中的蛋白表达水平也显著上调（P〈0．05）。结论：胰腺癌细胞株经培美曲塞诱导后，其组蛋白HIST1H2AC和HIST1H2BC在基因和蛋白表达水平上均出现上调，提示HIST1H2AC和HIST1H2BC基因可能与耐药有关。

英文摘要：

Objective： To investigate the expression of HIST1H2AC and HIST1H2BC genes in pancreatic cancer cell line Puta8988 treated with pemetrexed, and to verify the relationship between the genes and pemetrexedresistance. Methods： Pancreatic cancer cell line Puta8988 was treated with two doses of pemetrexed to get Puta8988 ＋ and Puta8988 ＋ ＋ with pemetrexed-resistance, and the expression levels of HIST1H2AC and HIST1H2BC and their genes in Puta8988, Puta8988 ＋ and Puta8988 ＋ ＋ were detected by qRT-PCR and Western blotting respectively. Results： Results detected by qRT-PCR showed that expression levels of HIST1H2AC and HIST1H2BC genes were significantly up-regulated in Puta8988 ＋ and Puta8988 ＋ ＋ （P 〈 0.05）. Results detected by Western blotting showed that expression levels of HIST1H2AC and HIST1H2BC were also significantly upregulated in those cell lines with pemetrexed-resistance （P 〈 0.05 ）. Conclusion： Expressions of HIST1 H2AC and HIST1 H2BC and their genes in pancreatic cancer cell line Puta8988 treated with pemetrexed are significantly upregulated, which implies that these genes possibly plays a role in pemetrexed-resistance of pancreatic cancer cell lines.