中文摘要：

目的在体外细胞实验中，观察二十碳五烯酸（EPA）联合顺铂（DDP）对肺腺癌A-549细胞株的抗增殖和诱导凋亡作用。方法选用A-549细胞株进行体外培养，用MTT比色法测定EPA对A-549细胞株的抗增殖作用。应用倒置显微镜、光学显微镜观察细胞凋亡形态学变化。应用TUNEL染色法检测细胞凋亡指数（AI）。结果实验结果表明，EPA在（60～120）μg／mL浓度范围内，于24、48、72、96h四个时间段对肺腺癌A-549细胞株均有抑制生长作用，且EPA与顺铂有协同抗癌作用，并表现出浓度、时间依赖性关系。当EPA浓度为60、80、100、120μg／mL时，细胞凋亡指数（AI）依次升高，分别为（81．52±1．96）％、（83．74±2．21）％、（85．30±2．38）％、（86．26±2．44）％，不同浓度组相比差异有统计学意义（P〈0．05）。结论体外实验中，EPA对A-549细胞生长有抑制作用，呈明显的量效和时效关系，且与顺铂有协同作用。

英文摘要：

Objective To observe the effects of anti-proliferation and inducing apoptosis on A-549 cell line induced by eicosapentaenoic acid （ EPA ） and its synergistic effect with cisplatin （ DDP ） in vitro. Methods A-549 cell line was cultured in vitro. MTT assay was used to determine the growth inhibitory effect of EPA on A-549 cells. Morphological changes of the apoptosis of A-549 cells were observed by using inverted microscope. TUNEL was used to detect cell cycle distribution and apoptotic rate of A-549 cells. Results The results of experiments showed that when A-549 cells were exposed to EPA for 24h, 48h, 72h and 96h respectively at final concentration of （ 60-120 ）μg/mL, the growth of cells was inhibited and showed in dose and time dependent manner. EPA combined with DDP showed significant synergistic anti-tumor effect on A-549 cells. When the concentration of EPA altered from 60 μ g/mL to 120μ g/mL, the apoptotic index （ AI ） of A-549 cells elevated accordingly by the method of terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling （ TUNEL ）, the apoptotic indexes were （ 81.52 ± 1.96 ） %, （ 83.74 ± 2.21 ） %, （ 85.30 ± 2.38 ） %, （ 86.26 ± 2.44 ） % respectively and showed statistical significance compared between different concentration （ P 〈 0.05 ）. Conclusion EPA inhibits growth of A-549 cell line in vitro in dose and time dependent manner; EPA combined with DDP shows significant synergistic anti-tumor effect on A-549 cell line.