中文摘要：

目的探讨羟基喜树碱（HCPT）联合多柔比星抑制肝细胞癌转移机制。方法以人肝癌细胞系HepG2及正常肝细胞LO2为实验对象,使用噻唑蓝（MTT）法比较多柔比星处理组、HCPT组及多柔比星联合HCPT组对肝癌细胞HepG2和正常肝细胞LO2的作用差异。荧光定量PCR检测E-selectin、VEGF、MMP-9、Bcl-2及Bax mRNA的变化。Westernblot检测E-selectin和MMP-9蛋白表达的变化。结果 MTT实验表明作用48 h后,1.5 mg.L-1多柔比星对肝癌细胞HepG2的细胞抑制率为16.58%,2 mg.L-1HCPT为39.92%,而HCPT（2 mg.L-1）联合多柔比星（1,1.5 mg.L-1）组分别为66.61%和74.47%。Real-time PCR检测表明E-selectin mRNA相对表达量多柔比星组为（52.13±0.58）,联合组为（93.41±8.43）、MMP-9 mRNA相对表达量多柔比星组为（26.49±0.11）,联合组为（6.48±0.05）。联合组Bcl-2及Bax表达与其他实验组比较均差异有显著性（P〈0.05）。结论 HCPT杀伤肝癌细胞HepG2的作用优于多柔比星,HCPT联合多柔比星抑制肝癌细胞生长的效应更加明显。HCPT联合多柔比星可以明显降低HCPT及多柔比星的药物浓度,减少这些药物对肝脏的细胞毒作用。

英文摘要：

Objective To explore the mechanism of killing effects against hepatoma cell by hydroxycamptothecine（HCPT） combined with doxorubicin（ADM）. Methods The growth difference between human hepatoma carcinoma cell（HepG2） and normal hepatic cell LO2 by 1.5 mg·L-1ADM,2 mg·L-1HCPT and two drugs combination,respectively,was observed by MTT method.The expression of E-selectin,VEGF,MMP-9,Bcl-2,Bax were examined by real-time PCR and E-selectin and MMP-9 were detected by Westernblot as well. Results It was shown that the growth rates of HepG2 treated by 1.5 mg·L-1 ADM and 2 mg·L-1 HCPT were 16.58% and 39.92%,respectively,while those treated by ADM（1 mg·L-1）and HCPT（2 mg·L-1） were 66.61%,ADM（1.5 mg·L-1） and HCPT（2.0 mg·L-1） were 74.47%.The expression of E-selectin mRNA was increased from（52.13±0.58）in ADM group to（93.41±8.43）in drug combined group.The expression of MMP-9 and VEGF mRNA was decreased from（26.49±0.11）in ADM to（6.48±0.05）in drug combination.The change of Bcl-2 and Bax were statistically significant in the experiment groups（P0.05）. Conclusion HCPT arrest the growth of HepG2 more effectively than ADM.While,HCPT combined with ADM kill more hepatoma cells than HCPT or ADM alone.Combined use of ADM and HCPT could reduce the dose and cytotoxicity of the single drug.