中文摘要：

目的观察携带人mda-7／IL-24的溶瘤腺病毒SG600IL24对各种不同转移潜能肝癌细胞HepG2、SMMC7721、MHCC97L和正常肝细胞L02的作用。方法将携带人mda-7／IL-24的溶瘤腺病毒SG600-IL24分别感染人肝癌细胞系HepG2、SMMC7721、MHCC97L和正常肝细胞L02，逆转录．聚合酶链式反应（RT-PCR）、酶联免疫吸附试验（ELISA）、Westernblot检测mda-7／IL-24基因和蛋白的表达，M1Tr观察肝癌细胞的生长抑制，Hoeehst33258和流式细胞仪（FCM）检测细胞的凋亡，流式细胞仪（FCM）检测细胞周期。结果RT-PCR和Westernblot显示mda-7／IL-24在肝癌细胞和正常肝细胞中高表达，ELISA提示细胞培养上清液中mda-7／IL-24蛋白也明显增加。MTT和流式细胞仪提示mda-7／IL-24能明显抑制肝癌细胞的生长（生长抑制率分别为75％±2．5％，86％±3．5％，72％±1．8％，HepG2：F=5．86，SMMC7721：F=7．98，MHCC97L：F=5．13，均P〈0．01），促进肝癌细胞的凋亡（凋亡抑制率分别为56．5％±4．0％，34．4％±2．0％，43．3％4-2．5％，HepG2：F=203．4，SMMC7721：F=130．5，MHCC97L：F=160．6，均P〈0．01），阻滞肝癌细胞在G2／M期（G2／M期阻滞分别为35．4％±4．2％，40．5％4-5．O％，42．0％±5．0％，HepG2：F=112．5，SMMC7721：F=133．2，MHCC97I．：F=145．5，均P〈0．01），而对正常肝细胞没有明显的促进凋亡和增殖阻滞作用。结论溶瘤腺病毒SG600-1124能特异性杀伤不同转移潜能人肝癌细胞和诱导凋亡，促进肝癌细胞增殖阻滞，而对正常肝细胞无明显促进凋亡和增殖阻滞作用。

英文摘要：

Objective To investigate the effect of oncolytic adenovirus vector SG600-IL24 expressing human melanoma differentiation associated gene-7 （mda-7/IL-24） on hepatocellular carcinoma cell lines with different metastatic potential of HepG2, SMMC7721, MHCC97L and normal liver cell line LO2. Methods The oncolytic adenovirus SG600-IL24 which carrying mda-7/IL-24 gene was transfected into hepatocellular carcinoma cell lines and normal liver cell line. The mRNA and protein expression of mda- 7/IL-24 in HepG2, SMMC7721, MHCC97L and L02 cell lines was confirmed by RT-PCR,ELISA assay and Western blot respectively. MTF assay and flow cytometry were used to study tumor cell proliferation and cell cycle in vitro. Hoechst33258 and flow cytometry were studied to indicate the apoptosis effects. Results It was confirmed by RT-PCR, ELISA assay and Western-blot that the exogenous mda-7/IL-24 gene was highly expressed in HepG2, SMMC7721, MHCC97L and L02 cell lines. MTT and apoptosis detection indicated that MDA-7/IL-24 can induce the growth suppression （the inhibition rate was 75% ±2. 5% ,86% ±3.5%, 72% ± 1.8% respectively, HepG2 ： F = 5.86, SMMC7721 ： F = 7.98, MHCC97L： F = 5.13, P 〈 0. 01 ）, and promotes apoptosis （ the apoptosis rate was 56. 5% ± 4. 0%, 34. 4% ± 2. 0%, 43.3%±2. 5% respectively, HepG2 ： F = 203.4, SMMC7721 ： F ： 130. 5, MHCC97L： F = 160. 6, P 〈 0.01 ） , blocks cancer cell lines at G2/M phase（the blocking rate was 35.4% ±4.2%, 40. 5% ±5.0%, 42. 0% ±5.0% respectively, HepG2：F = 112. 5, SMMC7721 ： F = 133.2, MHCC97L： F = 145.5, P 〈 0. 01 ） in different metastatic potential hepatocellular carcinoma cell lines but not in normal liver cell line. Conclusions Oncolytic adenovirus vector SG600-IL24 can selectively induce growth suppression, promote apoptosis in hepatocellular carcinoma lines in vitro but not in normal liver cell L02.