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中文摘要：
利用绿豆环氧水解酶催化（R，S）-对硝基苯乙烯氧化物水解，产物（R）-对硝基苯乙二醇的产率超过常规酶促拆分的极限产率（50％），表明发生了对映体会聚。采用绿豆粉剂和粗酶制剂考察了不同条件下的生物催化反应，结果表明，添加吐温-80作为分散剂对反应有促进作用。对该酶的固定化进行了初步研究，发现酶经硅藻土吸附固定化后稳定性显著增加。在克级规模制备实验中，产物的总产率达73．3％；产物经一步重结晶，得到了光学纯度大于99％的（R）-对硝基苯乙二醇。
英文摘要：
Mung bean epoxide hydrolase was used for enzymatic preparation of （R）-p-nitrophenyl glycol by enantioconvergent hydrolysis of p-nitrostyrene oxide. Enzyme forms and reaction conditions were examined, and an efficient process and high product yield were obtained for this biocatalyst. Tween-80 was found to be an ideal dispersant. Various immobilized biocatalysts were prepared by different methods, and the biocatalyst adsorbed on diatomite powder showed better stability. Gram-scale preparation was performed, giving a yield of 73,3 % and an optical purity higher than 99 % ee,
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