中文摘要：

目的探寻新型脱细胞骨基质（Acellular Bone Extracellular Matrix,ABECM）材料及脱细胞骨基质-壳聚糖（Chitosan,CS）复合多孔支架的制备方法,并对其性能进行评价。方法采用Triton X-100、十二烷基硫酸钠（Sodium Dodecyl Sulfate,SDS）的脱细胞方法对猪股骨进行脱细胞处理,应用溶液共混、冷冻干燥法制备脱细胞骨基质-壳聚糖复合支架。对ABECM材料进行定性分析,观察ABECM-CS复合多孔支架的形态学、孔隙率、力学性能和细胞-支架复合培养的相容性。结果 ABECM材料HE染色、Hoechest33258荧光染色均无细胞残留,茜素红染色、Ⅰ型胶原免疫组织化学染色、骨形态发生蛋白-2抗体染色阳性。扫描电镜显示支架具有多孔结构,孔径50-200μm,孔隙率为（53.50±4.23）%,力学测试支架干燥状态纵向压缩模量为（33.23±14.45）MPa,支架湿润状态下的压缩模量为（2.27±1.13）MPa。细胞-支架复合培养相容性良好。结论制备的ABECM-CS复合多孔支架去细胞彻底,保留了脱细胞骨基质主要成分,具备合适的孔径和孔隙率,细胞相容性良好,是理想的骨组织工程支架。

英文摘要：

Objective To study the method to prepare novel acellular bone extracellular matrix（ABECM） and acellular bone matrix-chitosan scaffolds and to evaluate their physicochemical properties.Methods A fresh porcine femur was treated with Triton X-100 and sodium dodecyl sulfate（SDS）,respectively.Acellular bone matrix-chitosan scaffolds were prepared with the solution-mixing and freeze-drying method.ABECM was qualitatively analyzed.Morphology,porosity,mechanical properties and biocompatibility of cultured ABECM chitosan scaffolds were observed with acellular Triton X-100 and SDS,respectively.Results The histological staining of ABECM showed that there were no cellular debris in the scaffolds and anti-collagen Ⅰimmunohistochemistry staining was positive.SEM scanning showed that the scaffolds had a porous structure with a pore diameter of 50-200μm.The porosity assay showed that the average porosity was 53.50%±4.23%.The mechanical assay showed that the compress module of dry and wet scaffolds was（33.23±14.45）MPa and（2.27±1.13）MPa,respectively.The cell adhesion test showed that the BMSC grew well on the scaffolds without any growth inhibition.Conclusion Acellular bone matrix-chitosan scaffolds can reserve most extracellular matrix after thorough decellularization,with a good pore diameter and porosity as well as a good biocompatibility,thus making it a novel cell-carrier in bone tissue engineering.