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日粮添加高水平硫酸锌和纳米氧化铜对鸡脏器组织锌铜沉积的影响
  • 期刊名称:中国农业科学, 2011, 44(23): 4874-4881
  • 时间:0
  • 分类:S[农业科学]
  • 作者机构:[1]青岛农业大学动物科技学院,山东青岛266109, [2]山东省农业科学院家禽研究所,济南250100
  • 相关基金:国家自然科学基金项目(30871894)
  • 相关项目:纳米氧化铜对肉鸡生物学作用及在鸡体内代谢规律的研究
中文摘要:

【目的】研究高锌(硫酸锌)常铜(纳米氧化铜)日粮对鸡脏器组织铜锌含量的影响,探讨Zn2+与纳米氧化铜在消化道吸收时的互作关系。【方法】选取1日龄SPF白莱航蛋用公雏120只,随机分为4组,每组5个重复。1组为对照组,饲喂基础日粮(以纳米氧化铜的形式添加铜8 mg.kg-1);2—4组为高锌组,以ZnSO4.7H2O的形式分别在基础日粮中添加锌250、500和1 000 mg.kg-1。【结果】日粮添加锌250—1 000 mg.kg-1,受试鸡肝、腺胃、十二指肠和空肠中锌和金属硫蛋白含量显著高于对照组(P〈0.01或P〈0.05),鸡十二指肠铜含量极显著高于对照组(P〈0.01)。日粮添加锌1 000 mg.kg-1时,空肠铜含量极显著高于对照组和日粮锌添加组(锌添加量分别为:250和500 mg.kg-1,P〈0.01)。试验至42 d,日粮添加锌500和1 000 mg.kg-1时,鸡肝脏铜含量极显著高于对照组和日粮锌添加组(锌添加量为:250 mg.kg-1,P〈0.01)。日粮锌水平与鸡肝脏、十二指肠、空肠和腺胃锌含量呈显著正相关(P〈0.05),与十二指肠、空肠铜含量呈显著正相关(P〈0.05),与肝脏铜含量呈正相关(P〉0.05),与十二指肠、腺胃和空肠金属硫蛋白含量呈正相关(P〉0.05)。肝脏金属硫蛋白含量和铜含量呈极显著正相关(P〈0.01);十二指肠、空肠金属硫蛋白含量和铜含量呈正相关(P〉0.05)。【结论】日粮添加高水平硫酸锌没有拮抗鸡对纳米氧化铜的消化吸收,纳米氧化铜在消化道可能是以纳米颗粒的形式吸收。

英文摘要:

【Objective】 In order to discover the interaction between Zn2+ and nano-CuO in the digestive tract of chickens,an experiment was conducted to determine the effect of high zinc(ZnSO4?7H2O) and normal copper(nano-CuO) diets on copper and zinc contents of visceral tissues in chickens.【Method】One hundred and twenty one-day-old White Leghorn SPF chickens were divided into 4 groups randomly,and five replicates for each group.The basal diet was supplemented with nano-CuO to supply the copper level ats 8 mg?kg-1.The chickens of control group were fed with basal diet.The chickens of trial groups were fed with diets supplemented with ZnSO4?7H2O to supply the zinc level at 250 mg?kg-1,500 mg?kg-1 and 1 000 mg?kg-1,respectively.【Result】The results showed that when the chickens were fed with diets containing zinc at 250 mg?kg-1,500 mg?kg-1 and 1 000 mg?kg-1,the zinc and metallotionein contents of liver,proventriculus,duodenum and jejunum were significantly higher than the control group(P0.01 or P0.05),and the copper contents of duodenum were significantly higher than the control group(P0.01).When the diets containing zinc at 1 000 mg?kg-1 were fed to chickens,the copper contents of jejunum were significantly higher than the control group and when the diets containing zinc at 250 mg?mg-1 and 500 mg?mg-1 were fed to chickens(P0.01).On 42nd day,when the chickens were fed with diets containing zinc at 500 mg?kg-1 and 1 000 mg?kg-1,the copper contents of liver were significantly higher than the control group and when the chickens were fed with diets containing zinc at 250 mg?mg-1(P0.01).The zinc levels of the diets had a remarkable positive correlation with zinc contents of liver,proventriculus,duodenum and jejunum(P0.05),had a remarkable positive correlation with copper contents of duodenum and jejunum(P0.05),and had a positive correlation with copper contents of liver(P0.05).There was a positive correlation between zinc levels of the diets and the met

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