中文摘要：

目的 利用基因芯片技术高通量筛选前列腺癌雄激素去势抵抗相关的基因,为研究雄激素去势抵抗进展及前列腺癌的分子机制奠定基础.方法 提取并纯化LNCaP及C4-2细胞的总mRNA,反转录合成荧光分子标记的cDNA,与基因芯片杂交;采用Genepix Pro6.0图像分析软件分析,筛选表达差异的基因;KEGG富集分析每个pathway中差异基因富集的显著性.结果 在表达谱芯片中筛选出417个差异表达的基因,包括301个C4-2细胞中表达上调的基因,116个表达下调的基因;KEGG富集分析显示与前列腺癌相关表达差异的基因有GF、EGFR、HSP、BAD、P21、E2F、Raf、CyclinE、PSA、PI3K等,其中EGFR、Raf、PSA表达差异最明显.结论 EGFR、Raf、PSA基因的表达异常可能在雄激素去势抵抗型前列腺癌的形成过程中发挥着重要的作用,本研究为前列腺癌去势抵抗进展的分子机制的探索提供了理论依据.

英文摘要：

Objective cDNA microarray was used to compare the gene expression profile associated with castration resistant prostate cancer （CRPC） of androgen sensitive LNCaP prostate cancer （PCa） cell line to the androgen resistant C4-2 PCa cell line,to identify the possible mechanism of castration resistant prostate cancer.Methods LNCaP and C4-2 cell lines were cultured under standard conditions.Total RNA was extracted and purified,and then reverse transcript to cDNA,hybridized according to the Agilent chip instructions.The fluorescent signals were analyzed and displayed by using Genepix Pro6.0 to find differential expressed genes.KEGG transcriptional domain coverage analyzed the differential expressed genes involved in signaling pathway.Results Comparing C4-2 and LNCaP cell lines,differential expressed genes are 417 in total,including 301 upregulated genes and 116 downregulated genes.KEGG transcriptional domain coverage revealed differentially expressed genes in PCa associated signaling pathway were GF,EGFR,HSP,BAD,P21,E2F,Raf,CyclinE,PSA,PI3K.Respectively,EGFR,Raf,PSA are the most significant differentially expressed genes in PCa associated signaling pathway.Conclusion 10 differential expressed genes in LNCaP and C4-2 cell lines are related to PCa associated signal pathway.EGFR,Raf and PSA may play an important function in the development of CRPC,this study provides the theory basis to explore the molecular mechanism of CRPC.