中文摘要：

目的：明确Lhx8在神经生长因子（nerve growth factors,NGF）促进海马神经干细胞向胆碱能神经元分化中的作用。方法：体外培养海马神经干细胞并应用NGF促进其向胆碱能神经元分化的过程中,将构建的Lhx8干扰慢病毒加入至培养液中,7 d后应用免疫荧光标记技术检测分化所得胆碱乙酰转移酶（choline acetyltransferase,Ch AT）和微管相关蛋白-2（microtubule-associated protein 2,MAP-2）双标阳性细胞。结果：在空白对照组中,仅检测到少量的Ch AT/MAP-2双标阳性细胞;在NGF组或是NGF联合阴性对照慢病毒组中则可检测到较多的Ch AT/MAP-2双标阳性细胞;在加入Lhx8干扰慢病毒的实验组中,分化所得的Ch AT/MAP-2双标阳性细胞数较NGF组或是NGF联合阴性慢病毒组明显减少。结论：Lhx8基因沉默抑制了NGF诱导的海马神经干细胞向胆碱能神经元的分化。

英文摘要：

Objective：To understand possible effects of Lhx8 in nerve growth factors（ NGF）-induced cholinergic neuronal differentiation of hippocampal neural stem cells（ NSCs） in vitro.Methods：Hippocampal NSCs were cultured in vitro and NGF was used to promote cholinergic neuronal differentiation,the Lhx8 interference lentiviral vectors（ Lv-Lhx8-RNAi） were added into culture medium.7 d later,choline acetyltransferase（ Ch AT） and microtubule-associated protein2（ MAP-2） double-labeled cells were detected by using immunofluorescence.Results：In control group,only a little Ch AT / MAP-2 double-labeled cells could be detected;in NGF group or NGF combined negative control group,more Ch AT / MAP-2 double-labeled cells were detected;after Lv-Lhx8-RNAi transfection,the number of Ch AT / MAP-2 doublelabeled cells in Lv-Lhx8-RNAi group was less than NGF or NGF combined with negative control group.Conclusion：Lhx8 gene silence inhibited NGF-induced cholinergic neuronal differentiation of hippocampal NSCs in vitro.