中文摘要：

目的 比较纳米铅和常规铅暴露对生长发育期仔鼠海马及皮质的氧化损伤作用。方法 将30只健康妊娠SPF级SD大鼠随机分为3组,分别为对照（生理盐水）组、纳米铅（10 mg/kg）组和常规铅（100 mg/kg）组,每组10只。采用经口灌胃方式进行染毒,自母鼠妊娠至仔鼠断乳（PND21）。记录仔鼠的出牙时间、睁眼时间、翻正反射时间、爬行时间、张耳时间、断崖回避时间,采用Morris水迷宫对PND21仔鼠进行学习记忆能力测试,分别于PND1、PND21检测仔鼠海马和皮质中的铅、活性氧（ROS）、蛋白质糖基化终产物（AGEs）含量和抑制羟自由基能力。结果 与对照组比较,纳米铅及常规铅暴露后仔鼠翻正反射时间、爬行时间及张耳时间延长,学习记忆能力降低;皮质、海马中铅、ROS和AGEs含量增高,而抑制羟自由基能力降低,差异均有统计学意义（P〈0.05）。与常规铅组比较,纳米铅组PND21仔鼠皮质和海马中ROS水平均升高,PND1时纳米铅组仔鼠海马抑制羟自由基能力下降,纳米铅暴露组PND21仔鼠海马和皮质中AGEs含量增高,差异均有统计学意义（P〈0.05）。结论 纳米铅和常规铅染毒可导致仔鼠生长发育期迟缓及皮质、海马的氧化应激,且随着染毒时间延长,纳米铅暴露致仔鼠皮质和海马氧化损伤程度高于常规铅暴露,这可能与纳米材料的特性有关。

英文摘要：

Objective To know the difterence of oxidative damage of hippocampus and cortex induced by nano-lead and lead exposure in newborn rats. Methods Thirty SD pregnant female rats were divided into three groups, nano-lead group and lead group and control group,and treated with nano-lead at 10 mg/kg, with lead at 100 mg/kg lead and saline respectively, through gavage, until postnatal day 21.The growth and development status of rats after birth were monitored. Morris water maze test was used to detect learning and memory performance of offspring. The lead content of hippocampus and cortex of offspring was determined by ICP-MS. Flow cytometry was applied to determine the ROS content. ELISA was used to detect the AGEs content. Meanwhile the ability of restraining the hydroxy radical produced in hippocampus and cortex were measured. Results Compared with the control group, significant delay in righting reflex,crawl and ear unfolding, reduced ability of learning and memory were found in offspring in nano-lead group and lead group on PND 21. Compared with the control group, the lead content,the ROS levels,the AGEs content of hippoeampus and cortex increased, and the ability of anti-hydroxyl radical production reduced in offspring in nano-lead and lead exposure group on PND 1 and PND 21 （P〈0.05）. Compared with lead exposure group, the ROS levels in cortex and hippocampus significantly increased on PND 21, and the ability of anti-hydroxyl radical production in hippocampus significantly decreased on PND 1, the AGEs content in cortex and hippocampus significantly increased on PND 21, in offspring of nano-lead exposed groups （P〈O.05）. Conclusion Both of nano-lead and lead exposure can cause the rat growth delay and the oxidative stress in hippocampus and cortex. Moreover,with the increase of exposure time,the oxidative damage induced by nano-lead is more severe than that of lead, which may be related to the properties of nano-materials.