中文摘要：

目的 探讨铅和高脂饮食联合暴露对大鼠学习记忆功能损伤及海马组织炎症因子的影响.方法 无特定病原体级成年雄性SD大鼠随机分为对照组、高脂饮食组、铅暴露组和联合暴露组,每组10只.对照组大鼠予基础饲料喂养,高脂饮食组大鼠予高脂饲料喂养,铅暴露组大鼠予基础饲料喂养同时饮用质量浓度为400 mg/L的醋酸铅饮用水;联合暴露组大鼠予高脂饲料喂养同时饮用质量浓度为400 mg/L的醋酸铅饮用水.隔周测量大鼠体质量.暴露9周后,采用Morris水迷宫实验检测大鼠的学习记忆能力,采用酶标仪检测血清中总胆固醇、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL）、高密度脂蛋白胆固醇（HDL）的水平,采用电感耦合等离子体-质谱仪检测海马组织中铅水平,采用酶联免疫吸附实验检测海马组织中肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-4、IL-6和干扰素-γ（IFN-γ）的水平.结果 自第3周起,高脂饮食组与联合暴露组大鼠的体质量均高于对照组（P〈0.05）,联合暴露组体质量高于铅暴露组（P〈0.05）.与对照组比较,高脂饮食组、铅暴露组及联合暴露组大鼠逃避潜伏期均延长（P〈0.05）,穿越平台次数均下降（P〈0.05）;联合暴露组大鼠逃避潜伏期分别较高脂饮食组及铅暴露组延长（P〈0.05）.分别与对照组和铅暴露组比较,高脂饮食组和联合暴露组大鼠血清TG和LDL水平均升高（P〈0.05）,HDL水平均降低（P〈0.05）.分别与对照组和高脂饮食组比较,铅暴露组和联合暴露组大鼠海马组织中铅水平均升高（P〈0.05）.与对照组比较,其余3组大鼠海马组织中TNF-α、IL-6、IL-1β、IFN-γ水平均升高（P〈0.05）,铅暴露组和联合暴露组大鼠海马组织中IL-4水平均升高（P〈0.05）.联合暴露组大鼠海马组织中IL-1β水平分别高于高脂饮食组和铅暴露组（P〈0.05）.结论 铅和高脂饮食联合暴露?

英文摘要：

Objective To investigate the effects of lead exposure and high fat diet on the hippocampal inflammatory factors and learning-memory in rats.Methods A total of 40 specific pathogen free male SD rats were randomly divided into control group,high fat diet group,lead exposure group,combine exposure group,10 rats in each group.Rats in control group were given regular diet and rats in high-fat diet group were given high-fat diet.Rats in lead exposure group were given regular diet and water with 400 mg/L lead acetate.Rats in combine exposure group were given high fat diet and water with 400 mg/L lead acetate.Body weight was measured every other week.The exposure period was 9 weeks.Morris water maze was applied to measure the learning-memory.The content of total cholesterol,triglyceride （TG）,low density lipoprotein cholesterol （LDL） and high density lipoprotein cholesterol （HDL） in serum were detected by using microplate reader.The lead content of hippocampus was detected by inductively coupled plasma mass spectrometer.Enzyme-linked immuno sorbent assay was used to detect the content of tumor necrosis factor-α （TNF-α）,interleukin （IL）-1β,IL-4,IL-6 and interferon-γ （IFN-γ）.Results Beginning from the third week,the body weight of rats in high fat diet and combine exposure group were significantly higher than that in control group （P〈0.05）.In addition,the body weight of rats in combine exposure group were higher than that in lead exposure group （P〈0.05）.Escape latency and the frequency of crossing platform of rats in high fat diet group,lead exposure group and combine exposure group were significant changed compared with those in control group （P〈0.05）.The escape latency of rats in combine exposure group increased compared with those in high fat diet group and lead exposure group （P〈0.05）.In addition,serum TG and LDL content in high-fat diet group and combine exposure group increased and HDL decreased compared with the control exposure group and lead exposure group （P?