中文摘要：

目的探讨小G蛋白超家族成员RhoA对食管癌Eca-109细胞血管内皮生长因子（VEGF）表达的调控作用。方法食管癌Eca-109细胞转染过表达RhoA质粒（V14RhoA）或沉默表达RhoA质粒（shRhoA）后,采用Western blotting和酶联免疫吸附试验（ELISA）法分别检测其细胞内外VEGF蛋白表达,Western blotting和Real-time PCR分别检测Eca-109细胞转染V14RhoA和shRhoA后p53和VEGF表达的情况。结果 Western blotting和ELISA法检测显示,Eca-109细胞转染V14RhoA后细胞内、外VEGF表达水平升高,而转染shRhoA后VEGF的表达被显著抑制。Western blotting和Real-time PCR检测显示,Eca-109细胞转染V14RhoA后p53表达显著降低,而VEGF表达显著升高（P〈0.05）。结论食管癌Eca-109细胞中RhoA表达的变化可以引起细胞内外VEGF水平的变化,并且RhoA可通过抑制p53表达从而增加对VEGF的调控。

英文摘要：

Objective To explore the effect and mechanism of RhoA on vascular endothelial growth factor（ VEGF） expression and regulation in esophageal carcinoma cell line Eca-109. Methods Eca-109 cells were transfected by RhoA up-regulating plasmid（ V14RhoA） or down-regulating plasmid（ shRhoA） and VEGF expression level was examined by Western blotting and ELISA assay.The effect of RhoA on p53 expression was examined by Western blotting and real-time PCR. Results Western blotting and ELISA assays showed that in Eca-109 cells VEGF protein expression was increased or decreased with up-regulating or down-regulating RhoA.Western blotting and real-time PCR assays showed that increased RhoA could inhibit p53 expression and then promote VEGF expression. Conclusion In Eca-109 cells,RhoA can regulate VEGF expression,and RhoA promotes VEGF expression through inhibiting p53 expression.