中文摘要：

表皮蛋白与几丁质结合构成抵御外界不良环境的昆虫角质层，在昆虫的生长发育及蜕皮硬化中具有重要的作用。为了探讨表皮蛋白基因在褐飞虱Nilaparvatalugens生长发育中的功能，本研究根据褐飞虱转录组测序信息，对其中1个预测为编码表皮蛋白的Unigene36450序列进行了克隆，并应用荧光定量PCR和RNA干扰（RNAi）技术分别对该基因的表达规律和功能进行了研究。结果表明：克隆的Unigene36450全长cDNA开放阅读框长585bp，编码的蛋白含194个氨基酸，具有典型的表皮蛋白R＆R保守结构域，命名为NIlCP。转录水平的时序表达分析发现，NIlCP仅在褐飞虱若虫期表达，且在3龄若虫体内表达量最高，提示该基因编码的蛋白属于幼虫表皮蛋白。RNA干扰结果显示，取食dsNllCP的1龄末2龄初（孵化后第3天）若虫在干扰6d和8d时，NIlCP基因的表达量分别较取食dsGFP的对照组下降58．8％和45．6％，差异极显著（P〈0．01）；干扰后部分褐飞虱若虫因蜕皮不完全死亡，干扰5d的存活率较对照下降26．7％。本研究结果提示，NIlCP与褐飞虱若虫的生长发育及蜕皮相关，可以作为褐飞虱防治的潜在靶标基因。

英文摘要：

Insect cuticular protein plays a vital role in insect development and molting sclerosis, by combining with chitin as a barrier to the external environment. To study the function of insect cuticular protein in the rice brown planthopper, Nilaparvata lugens, the full-length of Unigene36450 predicted to code an insect cuticular protein was amplified by RT-PCR and rapid-amplification of eDNA ends according to the RNA-seq analysis of transcriptome. Then the real-time quantitative PCR and RNAi experiment was conducted to explore the expression pattern and the function of NIICP. Bioinformatics analysis showed that Unigene36450 contains a 585 bp open reading frame encoding a protein of 194 amino acid residues, with a consensus region of R＆R consensus, so we named it NlICP. The transcripts of NUCP were detected only in the nymphal stage of N. lugens, and its expression reached the highest level in the 3rd instar nymph and then decreased with the nymphal development, suggesting that the coded protein of NlICP belongs to larval cuticular protein. RNAi analysis revealed that N. lugens nymph fed with dsNllCP was disturbed, and the expression levels of NUCP decreased by 58.8% and 45.6% after the continuous feeding for 6 and 8 d, respectively, significantly lower than those in the control group （P 〈 0. 01 ）. Some nymphs died because of incomplete molting after RNAi, and the survival rate decreased by 26.7% compared to the control group after continuous feeding for 5 d. The results suggest that NlICP is associated with N. lugens nymphal ecdysis and development, and may serve as a potential target gene for controlling IV. lugens.