中文摘要：

目的建立小鼠基因转录表达分析中内参基因的选择方法。方法以C57BL/6J和C3H/HeJ两个品系3个不同组织及2个不同发育阶段为研究对象,应用反转录实时定量PCR技术,评价GAPDH（glyceraldehyde-3-phosphate dehydrogenase）、HPRT1（hypoxanthine phosphoribosyl transferase）、B2M（β2-microglobulin）、PPIA（peptidylprolyl isomerase A）、ACTB（Actin-beta）和18S rRNA（18S ribosomal RNA）等6个看家基因在下丘脑、垂体与卵巢中mRNA水平的表达稳定性。结果 GeNorm统计分析表明,GAPDH和HPRT1表达最为稳定,PPIA等次之,B2M在不同组织和发育阶段中都几乎无表达。结论成功筛选到GAPDH和HPRT1两个稳定表达的看家基因,证实了小鼠基因表达转录分析中内参基因选择的必要性和可行性。

英文摘要：

Objective To evaluate the sellection of suitable internal reference genes for normalization of gene expressions in mouse.Methods The expression levels of six housekeeping genes including GAPDH,HPRT1,B2M,PPIA,ACTB and 18S rRNA in the hypothalamus,pituitary and ovarian tissues and two developmental stages of C57BL/6J and C3H/HeJ mouse strains were assessed by quantitative real-time PCR（qPCR）.Results The variation of expression levels was analyzed using geNorm program and demonstrated that GAPDH and HPRT1 were the most stable genes in different tissues at various developmental stages.PPIA,18S rRNA and ACTB were less stable,whereas B2M expression was almost undetectable in different tissues during development.Conclusions The successful selection of these two most stable housekeeping genes GAPDH and HPRT1 proves its necessity and feasibility,and provide the methodology for housekeeping gene selection as well.