中文摘要：

目的：探讨滤泡树突状细胞（FDC）增强HIV感染的机制。方法：应用细胞培养、transwell隔膜、ELISA等方法探讨FDC对淋巴细胞趋化及对HIV结合、进入淋巴细胞的作用。结果：FDC趋化的淋巴细胞（1.95±0.21）×10^5个高于PRMI1640培养液对照组（0.75±0.2）×10^5个,P〈0.05;FDC对HIV与淋巴细胞的结合无影响,P〉0.05;FDC或FDC培养上清使HIV进入淋巴细胞（203.3±31.0pg/ml,111.7±29.4.0pg/ml）的量高于对照组（64.0±1.0pg/ml）,P〈0.05。结论：FDC可能通过趋化淋巴细胞、促进HIV进入淋巴细胞达到增进HIV在淋巴细胞内的感染。

英文摘要：

Objective：To investigate the mechanism of follicular dendritic cell （FDC）-mediated enhancement of human immunodeficiency virus （HIV） infection in vitro. Methods：Cell culture,transwell plate,and enzyme-linked immunosorbent assay were performed to study the role of FDC in chemotaxis of lymphocytes,combination of HIV and lymphocytes,and HIV entry into lymphocytes. Results：The number of lymphocytes with FDC chemotaxis was higher than that in control group [（1.95±0.21）×10^5 vs. （0.75±0.2）×10^5,P 〈 0.05]. No effect of FDC on the combination of HIV and lymphocytes was found. FDC and FDC culture supernatant enhanced HIV entry into lymphocytes,and compared with control group,the levels of HIV-1 p24 antigen were significantly higher in FDC group （203.3±31.0 pg/ml） and FDC supernatant group （111.7±29.4.0 pg/ml）. Conclusion：FDC may enhance HIV infection by promoting the chemotaxis of peripheral lymphocytes and HIV entry into lymphocytes.