中文摘要：

目的：探索大鼠膀胱Cajal间质细胞（ICC）的分离和培养方法，为进一步研究其在膀胱中的作用提供条件。方法：取大鼠的膀胱组织，采用Ⅱ型胶原酶酶解法分离细胞，将细胞悬液接种于含50ng／ml SCF、15％（v／v）FBS的DMEM培养基中，进行培养。用c-kit特异性杭体标记细胞，免疫荧光鉴定ICC细胞。结果：培养8小时后的ICC贴壁良好，并保持其固有特征：两个长的突起，多个短的侧突，胞体小，核大，c-kit抗体荧光染色阳性。结论：酶解法分离大鼠膀胱ICC并培养成功。

英文摘要：

Objective： To investigate the role of the interstitial cells of Cajal （ICC） in rat bladder, the techniques of isolation and culture of ICC were explored from the rat bladder. Methods： The bladder tissue of rat was dissected. Collagenase II was used to isolate the ICC. The cells were suspended and cultured in DMEM medium containing 50ng/ml SCF and 15% （v/v） FBS. The c-kit antibody was applied to label ICC and to distinguish them from smooth muscle cells. Results： ICC adhered well after cultured for 8 hours. ICC in culture maintained the intrinsic characteristics： two long processes, multiple short lateral processes, large nuclei and small cell body. Fluorescent staining with c-kit antibody confirmed that the cultured ICC was c-kit positive. Conclusions： ICC has been isolated by enzyme digestion method from rat bladder and cultured successfully.