中文摘要：

目的：探讨孕18周胎儿膀胱是否有ICC样细胞的分布，为人膀胱ICC样细胞发育研究提供依据。方法：制备孕18周胎儿膀胱组织冰冻切片，通过c-kit多克隆抗体和平滑肌肌动蛋白单克隆抗体免疫荧光双标，激光共聚焦显微镜观察检测胎儿膀胱组织内ICC样细胞分布情况。结果：孕18周胎儿膀胱组织发现具有纺锤形的细胞体、椭圆形细胞核和突触等典型特征的ICC样细胞分布，位于平滑肌肌柬边缘和肌柬间，散在分布，未连接成网络。结论：首次报道人膀胱ICC样细胞在孕18周胎儿时已经发育。

英文摘要：

Objective： To detect whether there are interstitial cell of Cajal （ICC）-like cells in bladder of 18-week-old foetus, which may be a basement for the further study on the development of ICC-like cells in human bladder. Methods： Cryosections of bladder tissue of a 18-week-old foetus were labeled with primary（polyclonal antibody of C-kit （Anti-c-kit））and fluorescent secondary（monoclon- al antibody of smooth muscle actin （anti-SMA））antibodies, and imaged using confocal laser scanning microscopy to examined the distri- bution of ICC-like cells in the bladder tissue. Result： We found that there are ICC-like cells, which have atractoid bodies,ellipsoidal nucleus and synapses, in lhe bladder tissue of the 1 g-week-old foetus. It was most often seen at the edge of smooth muscle bundles and between smooth muscle bundles. They distribute independently without forming a network. Conclusion： We found the ICC-like cells in human bladder have developed in foetus as early as 18 Weeks old at first time.