中文摘要：

目的采用HPLC-ELSD法同时测定血塞通注射液（三七）中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd。方法采用Venusil XBP C18（250 mm×4.6 mm,5μm）色谱柱,流动相为乙腈-水梯度洗脱,体积流量1 mL/min,柱温30℃,雾化管温度36℃,漂移管温度70℃,载气压力25 psi（1 psi=6.895 kPa）。结果三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd分别在2.78～27.8μg/mL,9.24～92.4μg/mL,0.384～3.84μg/mL,5.55～55.5μg/mL,1.904～19.04μg/mL呈良好线性关系;血塞通注射液中三七皂苷R1、人参皂苷Rg1、Re、Rb1、Rd平均回收率（n=6）分别为97.60%、100.71%、98.14%、100.94%、99.69%。结论该方法简便、准确、分离好,灵敏度高,重复性好,无干扰,可用于血塞通注射液的质量评价及三七制剂的质量控制。

英文摘要：

AIM To establish a method for simultaneously determining notoginsenoside R1 and ginsenoside Rg1,Re,Rb1,Rd in Xuesaitong Injection（Notoginseng Radix et Rhizoma） by HPLC-ELSD.METHODS A Venusil XBP C18 column（4.6 mm × 250 mm,5 μm） was used with the mobile phase of acetonitrile-water for notoginsenoside R1,ginsenoside Rg1,Re,Rb1,Rd.The flow rate was 1.0 mL / min at 30 ℃.The temperature of driti tube was 70 ℃ and the nebulizer nitrogen flow was 25 psi（1 psi = 6.895 kPa）.RESULTS The linearities of notoginsenoside R1,ginsenoside Rg1,Re,Rb1,Rd were in the ranges of 2.78-27.8 μg / mL,9.24-92.4 μg / mL,0.384-3.84 μg / mL,5.55-55.5 μg / mL and 1.904-19.04 μg / mL,respectively.The average recoveries were 97.60%,100.71%,98.14%,100.94% and 99.69%,respectively.CONCLUSION The method is simple,reliable and precise,and could be used for quality control of the injection.