中文摘要：

学习在 uropathogenic Escherichia 关口 i (UPEC ) 和 uroepithelial 房间之间的相互作用在阐明是重要的尿道感染的致病。在这研究，非洲的绿猴子肾房间( Vero )，人的肾癌房间( Ketr-3 )和膀胱，癌房间( EJ )被 UPEC132 感染，从天津孤立的一个临床的种类，中国，并且被比较让他们的能力由这个种类允许坚持和侵略。结果表明所有这些房间线能被 UPEC132 依附并且入侵。为 Vero ， Ketr-3 和 EJ 房间的坚持率是( 49.20 ± 7.55 )%，( 55.22 ± 4.09 )%并且( 73.20 ± 5.26 )%，分别地并且侵略频率是( 2.61 ± 0.32 ) ×1 0 ⁻³,( 3.00 ± 0.34 ) ×1 0 ⁻³并且( 3.25 ± 0.20 ) ×1 0 ⁻³,分别地。统计分析证明为 EJ 房间的坚持率比那些显著地高因为另外的二根房间线(P【0.05 ) ，和为 EJ 和 Ketr-3 房间的侵略频率没有统计差别(P】0.05 ) ，但是为 Vero 房间(P【0.05 ) 比那高。三根房间线被使用间接免疫荧光为 UPEC 的 P pili 为受体检测。结果证明受体在所有房间线的表面上存在，并且最高的分发在 EJ 房间的表面上被发现。另外，由 recombinant UPEC132/pSELECT-GFP 的 EJ 房间的侵略能直接用共焦的显微镜学被设想。这些数据强烈含有 EJ 房间能被 UPEC132 更容易比另外的房间感染，并且能因此为 UPEC 感染的进一步的调查用作一个好试验性的目标。

英文摘要：

Studying the interaction between uropathogenic Escherichia coil （UPEC） and uroepithelial cells is important in elucidating the pathogenesis of urinary tract infection. In this study, the African green monkey kidney cells （Vero）, human kidney carcinoma cells （Ketr-3） and bladder carcinoma cells （EJ） were infected by UPEC132, a clinical strain isolated from Tianjin, China, and were compared for their capacities to allow the adherence and invasion by this strain. The results revealed that all these cell lines could be attached and invaded by UPEC132. The adherence rates for Vero, Ketr-3 and EJ cells were （49,20 ±7.55）%, （55.22 ±4.09）% and （73.20 ±5.26）%, respectively, and invasion frequencies were （2.61 ±0.32）×10^-3, （3.00 ±0.34）×10^-3 and （3.25 ± 0.20）×10^-3, respectively. The statistical analysis showed that the adherence rate for EJ cells was significantly higher than those for the other two cell lines （P〈0.05）, and the invasion frequencies for EJ and Ketr-3 cells had no statistical differences （P〉0.05） but were higher than that for Vero cells （P〈0.05）. Three cell lines were detected for the receptors for P pill of UPEC by using indirect immunofluorescence. The results showed that receptors existed on the surfaces of all cell lines, and the highest distribution was found on the surface of EJ cells. Additionally, the invasion of EJ cells by recombinant UPEC132/pSELECT-GFP could be directly visualized using confocal microscopy. These data strongly implicated that EJ cells could be more easily infected by UPEC132 than the other cells, and thus could serve as a good experimental target for further investigation of UPEC infection.