中文摘要：

目的：研究致肾盂肾炎大肠埃希菌（UPEC）132对细胞的粘附和侵袭能力。方法：对比致病菌株UPECl32及无菌毛代表菌株E．coliK-12p678—54对Vero、Ketr-3及EJ细胞的粘附率、粘附指数和侵袭指数。结果：E．coliK-12p678—54对此3种细胞无粘附无侵袭，而UPEC132对其有明显作用，可致细胞形态明显改变直至死亡。UPEC132对Vero、Ketr-3及EJ细胞的粘附率分别为（61．44±3．21）％、（55．22±4．09）％和（58．67±5．12）％，差别无统计学意义；对3种细胞的粘附指数分别为1．44±0．06、1.74±0，09和2．27±0．18，有显著性差异（P〈0．05）。UPEC132对EJ和Ketr-3细胞的侵袭指数分别为（3．25±0．20）×10^-3。和（3．00±0．34）×10^-3，两者之间无统计学差异，但均高于对Vero细胞的侵袭指数[（2．61±0．32）×10^-3，P〈0．05]。结论：UPEC132对Vero、Ketr-3、EJ细胞均有粘附和侵袭能力，其中对EJ细胞的粘附能力最强，侵袭力也较Vero细胞强，可利用该细胞深入研究UPEC132的毒力及致病机制。

英文摘要：

Objective： To study the adherence and invasion of host cells by uropathogenic Escherichia coli （UPEC）132. Methods： Pathogenic UPEC132 and nonfimbrial strain E.coli K-12 p678-54 were employed to adhere and invade Veto, Ketr-3 and EJ cells. The adhesion rate, adhesion index and invasion index of above three cell lines by both strains were calculated and compared. Results： E.coli K-12 p67854 did not adhere and invade tested cells, while UPEC132 caused not only morphological changes but also cell death. The adhesion rates of Vero, Ketr-3 and EJ cells by UPECI32 were （61.44±3.21）%, （55.22± 4.09） % and （58.67±5.12） % respectively without statistical differences, but the adhesion indexes of these cell lines were 1.44±0.06, 1.74±0.09 and 2.27±0.18 respectively with statistical differences （P〈0.05）. The invasion indexes of EJ and Ketr-3 cells by UPEC132 were （3.25±0.20）×10^-3 and （3.00±0.34）×10^- 3, and both of which were significantly higher than that of Vero cells [（2.61 ±0.32）×10^-3,p〈0.05].Conclusion： UPEC132 can adhere and invade Vero, Ketr-3 and EJ cells. Since EJ cell is more prone to be adhered by UPEC 132 than the other two, it can be selected for further study on the pathogenic mechanism of UPEC.