中文摘要：

目的 探讨端粒酶催化亚基（hTERT）启动子调控p53基因在膀胱肿瘤细胞T24中表达对细胞的影响。方法 将成功构建的phTERT-p53载体，瞬时转染膀胱肿瘤T24细胞；应用流式细胞仪观察细胞凋亡率变化，透射电镜下观察细胞凋亡形态学改变。结果 hTERT启动子调控p53基因表达使细胞凋亡率明显升高[24、48h凋亡率：实验组15．42％、36．57％；转染绿色荧光蛋白基因（GFP）组5．16％、8．19％；阴性对照组4．49％、7．18％]，电镜下观察到典型凋亡改变。凋亡指数达25％。结论 hTERT启动子能够激活phTERT-p53载体表达p53，使肿瘤细胞凋亡增加。

英文摘要：

Objective To explore the effect of expression of p53gene driven by human telomerase reverse transcriptase （hTERT） on human bladder turrnor cell T24. Methods The expression vector of p53 gene afforded by hTERT promoter was constructed and transiently transfected into T24 ceils, and the effect on apoptosis rate of tumor cells was detected by using flow cytometry （FCM） and morphological change under a transmission electron microscope. Results The expression of p53 driven by hTERT promotet increased apoptosis rate （apoptosis rate after 24/48 h：p53：15.42 %/36.57 % ;green fluorescence protein （GFP） ： 5.17%/8.19% ; negative control： 4.49%/7.18% ）. Typical morphological changes of apoptosis were observed under a transmission electronic microscope, and apoptosis index was 25 %. Conclusion The expression of p53 gene under the control of hTERT promoter can increase apoptosis rate of bladder carcinoma cells.