中文摘要：

为了从不同组织切片上获取某一特定细胞群或单一细胞进行分析研究,研制深度可控自动压电显微切割系统,以解决手工切割操作时间长、易疲劳、精度低等难题.在对超声振动切割进行理论分析的基础上,采用叠堆压电陶瓷作为振动发生装置.根据图像前后景差来检测跟踪切割针尖的实时位置信息,从而通过标定的X轴坐标的变化判断切割针和被操作物体表面的接触情况来获取深度信息.通过对不同条件下的显微切割实验进行分析,均表明该系统可实现将切割针置于被切割组织深度方向的任意位置;自动完成目标生物组织指定位置任意形状的切割,切割线细可达1.5 μm,理论上切割分辨率为50 nm,切割误差0.5 μm,可实现单细胞切割,并能自动实现目标组织切片的分离.明显提高显微切割操作的自动化程度和精度,证明该系统的优越性.

英文摘要：

In order to obtain a specific population of cells or a single cell from different tissue slice for the research, a depth-controlled and automated piezoelectric micro-dissection system was developed to solve problems of time consuming, easy fatigue, and low accuracy in the manual-dissection. On the basis of different gray values of foreground and background to detect and track the tip of the dissection needle＇ s position in real- time, and according to the change of the X axis to judge whether the dissection needle contact the target surface, the depth information was acquired. By analyzing the dissection experiments conducted in different conditions, it is shown that this proposed system can place the needle in any positions of the tissues, quickly scan the whole samples to determine the target area and realize the dissection in specify position with arbitrary shape of biological samples. The fineness of cutting line is 1.5μm, the resolution of cutting is 50 nm, and cutting error is 0.5μm, reaching the unicellular cut level. In addition, the separation of target tissue sections can be realized automatically. The method provides the improvement of the precision and efficiency of dissections, showing its superiority.