中文摘要：

制备了聚L-酪氨酸／半胱氨酸／纳米金／血红蛋白修饰Pt电极，并用循环伏安法和交流阻抗法对制备过程进行了表征。还用循环伏安法和计时电流法研究了修饰电极与H2O2的相互作用。结果表明，该电极对H2O2有明显的催化作用，电流与H2O2浓度在3．5×10^-7～2．0×10^-3mol／L范围内呈线性关系，检出限为1．0×10^-7mol／L。

英文摘要：

A poly （L-tyrosine）/cysteine/gold colloid/Hemoglobin modified electrode has been prepared. The electrochemical characteristics of the biosensor were studied by cyclic vohammetry and chronoamperometry. The modification process was characterized by electrochemical impedance spectroscopy （E IS） and cyclic vohammetry （CV）. The linear range for the determination of H2O2 is 3.5 × 10^-7 2.0 × 10^-3 mol/L with a detection limit of 1.0 × 10^-7 mol/L. The sensor exhibited high sensitivity, selectivity and stability. The method is applied to the determination of H2 O2 with satisfactory results.