中文摘要：

在玻碳电极（GCE）上,构造了一种以对氨基苯磺酸电聚合膜（PABSA）为基底,利用层层静电自组装技术固定多层天青Ⅰ（AI）和纳米金（nano-Au）制备的复合薄膜（nano-Au/AI）n,然后通过静电吸附辣根过氧化物酶（HRP）制得过氧化氢生物传感器[HRP/（nano-Au/AI）n/PABSA/GCE]。采用循环伏安法和计时电流法考察了该传感器的电化学性质,并且研究了该修饰电极对H2O2的催化还原作用。在优化的实验条件下,该传感器的响应电流与其浓度在3.5×10^-6～3.6×10^-3mol/L范围内呈现良好的线性关系,检出限为1.2×10^-6mol/L。该传感器的米氏常数为1.5 mmol/L,表明所固定的酶具有较高的生物活性。

英文摘要：

A sensitive amperometric biosensor for the determination of H2O2 has been constructed. Layer-by-layer selfassembly technique is used to form Azure Ⅰ and nano-Au muhilayer films （nano-Au/Al）n onto a poly （p-aminobenzene sulfonic acid）-modified glassy carbon electrode, then the positively charged horseradish peroxidase is successfully immobilized on the gold nanoparticles surface. The electrocatalytic behavior of the modified electrode to H2O2 was investigated by cyclic vohammetry and chronoamperometry. The experiments showed that the modified electrode had excellent electrocatalytic activity for the reduction of H2O2. The linear range of this biosensor is 3.5 × 10^-6 - 3.6 × 10^-3 mol/L with a detection limit of 1.2 × 10^-6 mol/L （S/N = 3）. In this experiment, HRP can retain a higher bioactivity with the Michaelis-menten Constant of 1.5 mmol/L.