中文摘要：

从玉米青贮中分离到一株共轭亚油酸生产菌（ANCLA01），发酵液中共轭亚油酸生成量为33．442μg／mL；菌株为革兰氏染色阳性杆菌且菌体短直、两端钝圆，不产芽孢；发酵时有乳酸生成，属于同型发酵B型并对底物要求特殊的乳酸杆菌；分子生物学鉴定结果表明，该菌株与Lactobacillus plantarum strain L5的16S rRNA碱基序列相似性达99．93％。兼顾该菌株传统鉴定方法，确定其为植物乳杆菌（Lactobacillus plantarum）。结果表明：ANCLA01菌株是青贮玉米中确产共轭亚油酸的植物乳杆菌。

英文摘要：

A bacteria, ANCLA01, which could convert linoleic acid to conjugated linoleic acid, was screened from maize Silage. The CLA yield of the fermentation broth was 33. 442 μg/mL; The germ was a Gram--positive rod while showed as short and straight, the beginnings and ends obtuse circle, no production of spore. Lactic acid was produced when it was been fermented. It＇s a kind of Bacillus acidi lactici which belonged to type B of homofermentation as well had the special requirement of substrate in medium. Moreover, 16S ribosomal RNA gene had 99. 93% sequence similarity with Lactobacillus plantarum strain LS. The results showed that the bacteria having the ability of bioconversion of linoleic acid into conjugated linoleic acid in silage.