中文摘要：

蛋白质糖基化是一种重要的蛋白质翻译后修饰方式,糖基化对蛋白质的结构和功能有着非常重要的影响。在血清或者组织提取液中,一些低浓度的糖蛋白/糖肽是具有高度临床灵敏性和特异性的生物标记物,这些生物分子可能对疾病发生机理探讨、疾病标记物发现及蛋白类新药开发提供重要信息。由于糖蛋白/糖肽的丰度低,从复杂的生物样品中高选择性富集糖蛋白/糖肽一直是糖蛋白组学的难点和重点。纳米结构的材料因其大比表面积、丰富的活性亲和位点和特殊结构,已经广泛应用于糖蛋白/糖肽的分离富集中。本文对基于金、Si O2、Ti O2、Fe3O4、金刚石和聚合物纳米粒子为载体的糖蛋白/糖肽分离富集方法的研究进展作了简要概述,并且阐明了糖蛋白/糖肽分离富集方法所面临的挑战,最后,对其未来发展方向做了展望。

英文摘要：

As one of the most important post-translational modifications,protein glycosylation has a significant effect on the structures and functions of proteins. Many low-abundance endogenous glycoproteins / glycopeptides in the serum or tissue extracts are biomarkers with higher clinical sensitivity and specificity,which could provide valuable information to explore the pathogenesis of many diseases,discover disease biomarkers and develop proteins based new drugs. However,it is still a great challenge for glycoproteomics to selectively separate and enrich the glycoproteins / glycopeptides from the complex biological samples due to their extremely lowconcentrations. Nanoparticle materials have been attracted much attention in the separation and enrichment of glycoproteins / glycopeptides due to their large surface to volume ratio,numerous affinity sites and unique structures. In this work,the recent progress for the separation and enrichment of glycoproteins / glycopeptidesbased on the nanoparticles,including gold,Si O2,Ti O2,nanodiamond and polymer nanoparticles are reviewed.Finally,the challenges and prospects for the methods of the separation and enrichment of glycoproteins /glycopeptides are briefly proposed.