中文摘要：

糖类化合物因其极性强，在反相色谱模式下保留较弱，因此常用亲水作用色谱（ HILIC）对其进行分离分析。本文以9种糖类化合物的混合物为研究对象，系统评价了其在Click TE-Cys亲水色谱柱上的保留行为，分别考察了流动相中有机相比例和盐浓度对其保留行为的影响。实验证明：9种糖类化合物按极性由小到大的顺序依次从Click TE-Cys色谱柱上被洗脱下来。随着有机相比例的增加，糖类化合物的保留增强；随着盐浓度的增加，除唾液酸外的糖类化合物的保留增强。用顶替-吸附液相相互作用模型模拟了糖类化合物在HILIC上的保留行为，采用保留方程ln k＝a＋bln CB＋cCB 描述HILIC的保留规律，对HILIC的保留值进行多元线性回归。结果表明糖类化合物在Click TE-Cys色谱柱上的保留行为符合HILIC的保留规律。

英文摘要：

Because of the strong polarity of carbohydrate compounds which have weak reten-tion in reverse-phase liquid chromatography （ RPLC） , hydrophilic interaction liquid chromatog-raphy （ HILIC） is suitable for the separation and analysis of the carbohydrate compounds. In this study, nine carbohydrate compounds were selected as the test probes to systematically evaluate the retention behavior of the carbohydrate compounds on a Click TE-Cys column with zwitterionic stationary phase. And the effects of the ratio of organic phase and salt concentra-tion in mobile phase on the retention behavior were investigated as well. The experiments proved that the nine carbohydrate compounds could be eluted in the order of their polarities from the Click TE-Cys column. With the increase of organic phase ratio, the retention of carbo-hydrate compounds was enhanced. As the increase of salt concentration, the retention of the carbohydrate compounds increased except sialic acid. Using displacement-adsorption liquid phase interaction model to simulate the retention behavior of the carbohydrate compounds under HILIC, the retention behavior of HILIC was described by the retention equation of ln k=a＋bln CB＋cCB, and the retention values of HILIC were analyzed by multiple linear regression. The results demonstrated that the retention behavior of carbohydrate compounds on the Click TE-Cys was in accordance with the retention regularity of HILIC.