目的:提取土人参叶多糖,测定其含量。方法:水提醇沉淀法提取多糖,苯酚-硫酸法显色,以葡萄糖标准液绘制标准曲线。结果:回归方程为Y=7.4625x-0.0484,相关系数r=0.9999,平均回收率98.7%,RSD=0.49%(n=5)。结论:土人参叶多糖含量为1.3%,方法简单易行,数据可靠,精密度和稳定性好。
Objective: Extraction of Artemisia capillaries polysaccharide, and content determination of polysaccharide from Talinum paniculatum leaves. Methods: Water extracting and alcohol precipitation extraction for content polysaccharides from Artemisia capillaries. Using phenol - sulfuric acid method to make them development color. With glucose as standard substance. Results: The regression equation for y = 7.4625x- 0.0484, the correlation coefficient r = 0. 9999. The average recovery rate is 98.7 %, RSD = 0.49 % ( n = 5 ). Conclusion: Artemisia polysaccharide Content is 1.3 % . The results show that the proposed method was easy, accurate, stability and precision weU.