中文摘要：

【目的】研究羊驼皮肤功能发生的规律和机理以及发现新的功能基因。【方法】用SMART技术构建白色青年羊驼皮肤cDNA文库，随机挑取13800个克隆进行规模测序。预处理后，获取高质量的表达序列。用CAP3对高质量序列进行拼接；BLAST对拼接序列进行同源比较，以注释功能；CLUSTER对已知序列进行聚类；根据标准基因词汇体系，并结合人组织基因表达谱分类标准，对具有注释信息的基因聚类进行分类，构建羊驼皮肤的基因表达谱。【结果】cDNA文库库容量达10^6PFU，从中获得高质量序列7286条（在GenBank中命名为ASCD）。拼接成5837条一致性序列，包括446条重叠群和5391条单一序列。1732条无相应注释，被认为是新基因。聚类成4968个单基因簇（Unigene）。该基因表达谱最显著的特点是代表基本转录和翻译系统以及细胞骨架结构的转录本的表达丰度处于最高水平，原胶原I型（ProcoI1agen typeI）等单个基因表达丰度处于较高水平。此外，还发现可能与羊驼毛质量、生长和毛色相关的基因。【结论】首次构建了羊驼皮肤cDNA文库和基因表达谱：获取的大量基因信息反映了青年期白色羊驼皮肤的生理特性和功能；该文库的构建是有效地发现新基因的理想资源。

英文摘要：

[ Objective ] The aim of this experiment is to study the mechanism of functions generation and to find the novel functional genes in the alpaca skin. [Method] The skin tissue was got from a white young alpaca. The cDNA library was constructed with SMART, from which 13 800 clones were selected at random to be sequenced. All sequences were treated to be high quality ones, which were assembled to be consensus sequences with CAP3, further aligned with BLAST to be annotated and clustered with CLUSTER. According to the Gene Ontology （GO） and classification standard of gene expression profile of human tissue, genes were classified to construct the gene expression profile. [Result] 7 286 high quality sequences were generated from the alpaca skin cDNA library with 10^6 PFU, which were deposited by dbEST in GenBank （GenBank name：ASCD）. With CAP3, 5 837 consensus sequences were assembled, including 446 contigs and 5 391 singleton. BLAST searches produced 1 732 novel genes representing new transcripts. Clustering analysis of the annotated sequences allowed 4 968 unigenes. The results showed that the significant characteristics were the highest level of the transcripts of transcription and translation system and higher level of some single genes such as Procollagen typeI. Additionally, some putative genes related to alpaca fleece quality, growth and color were found. [Conclusion] This is the first time to construct the alpaca skin cDNA library and its gene expression profile, from which much gene information reflecting the physiologic characters and functions of the white young alpaca skin were obtained. The construction of the cDNA library is a useful resource for finding new genes.