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人间充质干细胞抑制乳腺癌细胞增殖作用的实验研究
  • 期刊名称:乔玲,叶丽虹*, 张晓东*人间充质干细胞抑制乳腺癌细胞增殖作用的实验研究.南开大学学报自然科学版,2
  • 时间:0
  • 分类:R364.7[医药卫生—病理学;医药卫生—基础医学]
  • 作者机构:[1]南开大学生命科学学院,天津300071
  • 相关基金:国家自然科学基金(30570698);天津市科技发展计划应用基础研究重点项目(033801211)
  • 相关项目:Wnt信号传导途径在干细胞抑制肿瘤细胞中的作用
中文摘要:

应用人间充质干细胞作用肿瘤细胞,拟揭示间充质干细胞对肿瘤细胞的作用,为今后应用人间充质干细胞进行肿瘤细胞治疗奠定理论基础.应用胎儿真皮来源的Z3间充质干细胞和胎儿骨髓来源的BMMS-03间充质干细胞的条件培养液作用于乳腺癌MCF-7细胞,观察MCF-7细胞增殖和迁移能力的变化.结果显示,应用Brd U掺入分析和流式细胞仪测定等方法发现,在间充质干细胞条件培养液的作用下,MCF-7细胞的增殖受到了明显抑制;应用划痕实验和琼脂糖滴实验发现,MCF-7细胞的迁移能力也受到明显抑制.结果表明,人间充质干细胞对乳腺癌细胞的增殖和迁移具有抑制作用。

英文摘要:

To examine the effect of human mesenchymal stem cells (hMSCs) on human breast cancer MCF-7cells, the proliferation and migration of the cells were investigated. The hMSCs designated Z3 cells were established from human dermis tissues of a fetus aborted at 4 months gestation, immortalized by stable transfection of a pGRN145 plasmid containing the cloned hTERT gene. The MSCs designated BMMS-03 cells, derived from human fetal bone marrow in 4 month, were established without immortalization. In this study, we demonstrated that conditioned media from MSCs were able to decrease the proliferation and migration of MCF-7 cells in vitro. Proliferation of MCF-7 cells was decreased significantly by BrdU incorporation assay and flow cytometry assay after the treatment. Moreover, wound healing assay and agrose drop assay showed that the migration of MCF-7 cells was inhibited as well. Our finding demonstrated that hMSCs can directly inhibit breast cancer MCF-7 cells in vitro. Therefore, we conclude that hMSCs have a potential role in controlling proliferation and migration of solid tumor cells.

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