中文摘要：

干细胞移植治疗肿瘤具有重要的临床价值．应用人间充质干细胞条件培养液作用H7402肝癌细胞，拟探讨间充质干细胞对肿瘤细胞的抑制作用，为今后应用人间充质干细胞进行肿瘤细胞治疗奠定理论基础．应用胎儿真皮来源的Z3间充质干细胞和胎儿骨髓来源的BMMS-03间充质干细胞的条件培养液作用于H7402肝癌细胞，采用软琼脂克隆形成实验、流式细胞仪技术、基因芯片技术和免疫印迹技术观察H7402细胞的克隆形成、增殖和基因表达谱变化．结果显示，H7402细胞在间充质干细胞条件培养液作用下，克隆形成和增殖受到了明显抑制；基因芯片检测结果显示，H7402细胞在间充质干细胞条件培养液作用下有23个基因上调表达，17个基因下调表达，这些差异表达的基因与细胞的转录调控、新陈代谢、信号转导、细胞周期、应激反应和细胞粘附等功能相关．本实验结果表明，人间充质干细胞对H7402肝癌细胞的克隆形成和增殖具有抑制作用，并有多种基因的表达发生改变，这些基因表达的改变可能参与了对上述肿瘤细胞的抑制．

英文摘要：

Stem cell transplantation exhibits potential clinical values for the treatment of tumors. To investigate the underlying molecular mechanisms, we examined the effect of conditioned media from human mesenchymal stem cells （hMSCs） on human hepatoma H7402 cells. The designated hMSCs cells Z3 were established from human dermis tissues of an aborted fetus at 4 month gestation. The BMMS-03 cells were derived from the human fetal bone marrow at 4 month. H7402 cells were treated with the conditioned media collected from Z3 or BMMS-03 cells, then analyzed with colony-forming assay, flow cytometry, DNA microarray and Western blotting. The results showed that the conditioned media from hMSCs was able to decrease the number of colony-forming units of H7402 in vitro. The microarray data have revealed 23 genes were up-regulated and 17 genes were down-regulated, all of which are involved in the processes of transcription regulation, metabolism, cell cycle, signaling transduction, stress response and cell adhesion, and . the activity of NF-κB was down-regulated as confirmed by Western blot analyses. Our finding of hMSCs derived factors inhibit the proliferation of hepatoma cells suggested that depressing NF-κB played an important role in the control of malignant phenotypes.