目的:建立荔枝草提取物中高车前苷含量的高效液相(HPLC)测定方法。方法:HederaODS.2色谱柱(4.6mm×250mm,5μm),流动相甲醇-0.5%冰醋酸水溶液(50:50),柱温30℃,流速1mL·min^-1,检测波长335nm。结果:高车前苷在0.20—2.01μg呈良好的线性关系,平均回收率101.70%,RSD1.84%。结论:该方法简便、准确、专属性强,可以作为荔枝草提取物中高车前苷含量测定的一种有效方法。
Objective: To establish the assay method for determining content of homoplantaginin in extract of Salvia plebeia. Method: Hedera ODS-2 column was used with methanol-0.5% acetic acid (50: 50) as mobile phase; the column temperature was maintained at 30 ℃; the flow rate was 1 mL ·min^-1; detection wavelength was set at 335 nm. Result: Homoplantaginin showed a good linear relationship within 0.20-2.01 μg. The average of coefficient of recovery was 101.70% , RSD was 1.84%. Conclusion: This method is simple, accurate, specific, and can be considered as an effective method for determining content of bomoplantaginin in the extract of Salvia plebeia.