中文摘要：

采用不同剂量（0～10Gy）^60Coγ射线照射人外周血淋巴细胞，通过抽提mRNA，采用real-time PCR检测不同剂量照射后淋巴细胞中GADD45和CDKN1A基因mRNA表达水平的变化。筛选出最佳照射剂量（5Gy）后，用该最佳照射剂量照射淋巴细胞，检测不同时间点（0～72h）淋巴细胞中GADD45和CDKN1A基因mRNA表达水平的变化。结果表明，淋巴细胞中GADIM5和CDKN1A基因mRNA的表达水平随照射剂量的增加而增加，并呈现出剂量效应关系。在5Cy最佳剂量照射后，淋巴细胞GADIM5基因mRNA表达水平随时间的推移不断增高，在8h时达到最高，随后开始逐渐降低；淋巴细胞CDKN1A基因mRNA表达水平随时间的推移未见明显变化趋势。淋巴细胞系中GADD45和CDKN1A基因mRNA的表达水平与照射剂量均有较好的剂量效应关系，两者均有可能作为电离辐射的生物剂量计来估算受照剂量水平。

英文摘要：

This study is to investigate the changes of GADIM5 and CDKN1A gene mRNA expression induced by ^60Coγ-ray in lymphoblastoid cell line in vitro, and its probability of gene expression as a biodosimeter. Lymphoblastoid cell line was irradiated by ^60Coγ-ray with the dose of 0 - 10 Gy and cultivated for 8 hours, in order to screen the optimal dose. mRNA was extracted and real-time PCR method was used to detect the changes of PIG3 and GADIM5 gene mRNA expression in lymphoblastoid cells. Lymphoblastoid cells was cultivated for 0 -72 h after optimal dose irradiation. The changes of GADD45 and CDKNIA gene mRNA expression in lymphoblastoid cells in different time-point were detected. The resuits showed that GADD45 and CDKN1A gene expression in lymphoblastoid cells increased in a dose-dependent form. The peak expression of GADD45 was at 8 h after 5 Gy irradiation and began to decrease. The trend in change of CDKN1A gene expression was not notable.