中文摘要：

目的：研究电离辐射对体外胃腺癌细胞增殖、凋亡及周期的影响。方法：~（60）Coγ射线单次照射胃腺癌BGC-823细胞,照射剂量分别为0、2、4 Gy,利用MTT和克隆形成率实验检测各剂量组BGC-823的增殖能力;同时利用Annexin-V/PI双染法检测各剂量组BGC-823细胞的凋亡;用PI单染测定各剂量组BGC-823的细胞周期。结果：与0 Gy组相比,2、4Gy 60Coγ射线照射可显著抑制胃腺癌BGC-823细胞的增殖（P〈0.05）,诱导BGC-823细胞在照射后依次出现S期和G2/M期阻滞。4Gy 60Coγ射线照射后48h,凋亡细胞比例显著高于对照组（P〈0.05）。结论：60Coγ射线照射可抑制胃腺癌细胞BGC-823增殖、诱导胃癌细胞出现周期阻滞和细胞凋亡。

英文摘要：

OBJECTIVE：To study the effects of ionizing radiation on the proliferation,apoptosis and cell cycle of gastric adenocarcinoma cells in vitro.METHODS：After gastric adenocarcinoma BGC-823 cells were irradiated by 60Coγ-rays with a single dose of 0,2 or 4 Gy,the proliferation ability of BGC-823 cells in every dose group was evaluated by MTT and cloning efficiency assay.Apoptosis and cell cycle were further investigated by flow cytometry with Annexin-V/PI double staining and PI staining,respectively.RESULTS：Compared with the 0 Gy group,proliferation of gastric adenocarcinoma cells was inhibited significantly after doses of 2 and 4 Gyγ-rays irradiation（P〈0.05）. Furthermore,the cells were arrested in S then G_2/M phases afterγ-rays irradiation of 2 and 4 Gy.Finally,compared with 0 Gy group,the percentage of apoptotic cells in 4 Gy dose group increased significantly 48 h after irradiation（P〈0.05）. CONCLUSION：Radiation treatment of gastric adenocarcinoma BGC-823 cells with ~（60）Coγ-rays could inhibit cell proliferation,induce cell cycle arrest and promote apoptosis.