中文摘要：

为探讨电离辐射对人淋巴细胞线粒体复合物Ⅰ亚基因表达的影响,采用1、3、5、8和10Gy~（60）Co γ射线分别照射指数生长期的人淋巴细胞永生化细胞株,8h后利用逆转录PCR（RT-PCR）摸索各基因的最佳实验条件,进而利用实时荧光定量PCR（Real-timePCR）方法定量检测各基因表达变化,观察辐射对复合物Ⅰ基因表达影响的剂量效应关系;同时以5Gy~（60）Co γ射线照射人淋巴细胞后,分别于不同时间点（0.5、4、8、12、24、48和72h）,同样通过RT-PCR和Real-timePCR方法检测各基因表达的变化,观察其时效关系。结果表明,在mRNA水平上,线粒体复合物Ⅰ基因表达总体上调。剂量效应关系方面,ND2基因在8Gy剂量范围内呈现出随剂量增加基因表达增强的趋势;时效关系方面,7种亚基均在5Gy剂量照射后4h左右表达增强最显著,且大部分基因可持续高表达至48或72h左右。说明电离辐射可以诱导人淋巴细胞线粒体复合物Ⅰ基因表达的改变,表达总体上调。

英文摘要：

This study is to explore the changes of human mitochondrial complex Ⅰ（ND）genes expression induced by ionizing irradiation.Changes of human ND genes expression were detected by RT-PCR and real-time PCR 8 h after irradiation in human lymphoblastoid cell lines,which were exposed to 1—10 Gy ~（60）Co γ-rays.And the dose-effect relationships between expression changes of the genes and the doses were analyzed.The changes of these genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect.The results showed that the expression of ND were up-regulated comprehensively.A good dose-effect relationship was showed for ND2 and ND3,at 0—8 Gy and 0—3 Gy,respectively.For the time-effect after irradiation,the gene expression levels of all 7 ND genes up-regulated generally and the peak change time-point was 4 h after irradiation.Ionizing radiation might induce the changes of mitochondrial ND gene expression,and the ND gene expression levels are up-regulated.