引言 螺旋藻(Spirulina platensis)是一种丝状原核藻类,富含藻蓝蛋白、叶绿素、胡萝卜素及不饱和脂肪酸等多种生物活性成分。 叶绿素是绿色植物进行光合作用的主要色素,常见的有叶绿素a、叶绿素b等。叶绿素广泛应用于食品、医药等领域。
Chlorophylls were extracted by using ultrasonic from Spirulina platensis. Single factor examination and response surface analysis experiments were adopted to investigate the effects of extraction time, extraction solvent, solvent concentration, ratio of liquid to solid and extraction grade. The results showed that the optimal process parameters for this method were: extraction time of 56.5 min, ethanol concentration of 48.3% (vol) of ethanol/acetone solvent, and ratio of liquid to solid of 7.9 ml · g^-1. The optimized chlorophylls extraction yield was 1.28%. The comparison experimental results indicated that the yield of chlorophylls by ultrasonic extraction was higher than that obtained from conventional solvent extraction.