中文摘要：

目的 探讨神经-钙粘素（N-cadherin）、上皮-钙粘素（E-cadherin）及相关因子β-连环蛋白（β-catenin）在高、低级别人脑干胶质瘤中的表达及其意义.方法 采用免疫组化方法和RT-PCR法检测不同级别脑干胶质瘤N-cadherin、E-cadherin及β-catenin的表达.结果 N-cadherin蛋白在高、低级别脑干胶质瘤中表达差异无统计学意义（P＞0.05）,mRNA的表达在脑干胶质瘤中随着肿瘤级别的升高而增高,高、低级别间差异有统计学意义（t=2.711,P＜0.05）;E-cadherin蛋白和mRNA在高、低级别脑干胶质瘤中均表达缺失或减少,组间差异无统计学意义（P＞0.05）;β-catenin蛋白的表达在脑干胶质瘤中随着肿瘤级别的升高而增高,高、低级别间差异有统计学意义（P＜0.05）,mRNA的表达在脑干各级别胶质瘤中差异无统计学意义（P＞0.05）.结论 N-cadherin转录水平的过度表达及β-catenin蛋白水平的过度表达可能与人脑干胶质瘤的恶性进展有关,而E-cadherin转录和蛋白水平的表达下调可能与此无关.

英文摘要：

Objective To explore the expression and significance of N - cadherin, E - cadherin and β - catenin in human brainstem gliomas. Methods N - cadherin, E - cadherin and β - catenin proteins and mRNA were detected in 38 cases of brainstem gliomas using immunohistochemistry and RT - PCR Methods. Results There was no significant difference in the expression of N - cadherin between grades Ⅰ ～ Ⅱ and grade Ⅲ ～ Ⅳ （ P 〉 0. 05） . The expression of N - cadherin mRNA in human brainstem gliomas of grade Ⅰ ～ Ⅱ was significantly lower than that in grade Ⅲ～ Ⅳ （ t = 2. 711, P 〈 0. 05 ）. There was no significant difference in the expression of E - cadherin and E - cadherin mRNA between grade Ⅰ ～ Ⅱ and grade Ⅲ～ Ⅳ （ P 〉 0. 05 ）. The expression of β - catenin in human brainstem gliomas of grade Ⅰ ～ Ⅱ was significantly lower than that in grade Ⅲ ～ Ⅳ （ P 〈 0. 05 ）. As regard to β -catenin mRNA, there was no significant difference between grade Ⅰ ～ Ⅱ and grade Ⅲ ～ Ⅳ （ P 〉 0. 05 ）. Conclusions The over -expressionof N - cadherin and β - catenin may play an important role in the invasion and malignant progression of human brainstem gliomas.