中文摘要：

目的研究哺乳类动物雷帕霉素靶蛋白（mTOR）在自体移植静脉中表达的动态变化规律。方法Wistar大鼠64只，建立自体静脉移植模型，随机分为8组，分别在移植后6h，1、3d，1、2、4、6、8周切取移植静脉。逆转录PCR结合原位杂交研究移植血管中mTOR的mRNA表达，Western blot联合免疫组化检测mTOR蛋白产物表达的变化，同时检测增殖细胞核抗原（PCNA）。结果逆转录PCR扩增在静脉移植1～3d即出现mTOR mRNA表达增强，1～2周达到高峰，表达值分别为（48±18）％和（33±11）％，与其他组比较差异有统计学意义（P〈0．01），6～8周逐渐恢复正常。免疫组化及Western蛋白印迹均提示mTOR蛋白在移植3d表达明显增多，2～4周达到高峰，分别为（29±8）％和（31±6）％，与其他组比较差异有统计学意义（P〈0．01），8周恢复至正常水平。mTOR蛋白产物表达与PCNA表达呈正相关（r=0．756，P〈0．01）。结论mTOR在血管移植后的过程中被激活，与内膜增生关系密切，可能是防治移植血管狭窄、闭塞的干预靶点。

英文摘要：

Objective To study the expression of mammalian target of rapamycin （mTOR） in autogenous vein graft. Methods Autogenous vein graft model was established in 64 Wistar rats. Graft vein was harvested 6 hours, 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks and 8 weeks after transplantation, roTOR mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry methods were used to detect the expression of mTOR. PCNA expression was detected. Results The vein graft mRNA and protein expression of mTOR increased soon after transplantation, mTOR mRNA reached the peak 1 to 2 weeks after surgery [（48 ± 18）% and （33 ± 11）% respectively, P 〈0. 01 ） ]. Expression of mTOR protein reached the peak 2 to 4 weeks after surgery [ （29 ± 8 ） % and （31 ±6）% respectively, P 〈 0. 01 ]. There was a positive relationship between expression of roTOR and PCNA （ r = 0. 756, P 〈 0. 01 ）. Conclusion roTOR was activated in vein graft and roTOR may become a new target for the prevention and therapy of stenosis or obliteration after autogenous vein grafting.