中文摘要：

目的研究自体静脉移植后早期生长反应基因-1（Egr-1）表达的动态变化，探讨其在内膜增生（IH）中的作用。方法Wistar大鼠90只，建立自体静脉移植模型。术后分为1、2、6、24h，3、7、14、28、42d组，取正常静脉作为对照。应用原位杂交和RT—PCR方法检测Egr-1mRNA的表达，联合应用Western blot蛋白印迹和免疫组织化学方法检测Egr-1的蛋白表达。结果自体静脉移植后，Egr-1 mRNA和Egr-1蛋白的表达具有双相变化。移植后1h，Egr-1 mRNA基因表达值为2．21±0．85。6～24h时下降，7d时重新升高，28d达高峰为3．16±1．14。移植早期2h Egr-1蛋白的表达阳性率为31％±6％，24h至3d下降，28d达高峰为41％±8％。免疫组化显示：移植早期Egr-1蛋白阳性表达主要位于中膜血管平滑肌细胞（VSMCs）和单核细胞／巨噬细胞；移植后期Egr-1蛋白阳性表达主要位于新生内膜和中膜的VSMCs，同时部分内皮细胞也有表达。结论移植静脉内膜增生与Egr-1的激活及表达关系密切，因而与移植静脉内膜增生及狭窄有关.

英文摘要：

Objective To investigate the expression of early growth response gene-1 in autogenous vein graft in rats, and its role in intimal hyperplasia （ IH ）. Methods Autogenous vein graft model was established in 90 Wistar rats. Grafts were harvested at 1,2,6,24 hours,and 3,7,14,28,42 days after surgery. Normal veins serve as control. Egr-1 mRNA was measured by RT-PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of Egr-1. Results The expression of Egr-1 mRNA and Egr-1 protein experienced biphasic changes. At 1 hour, the value of gene expression of Egr-1 mRNA was 2. 21 ±0. 85, declining at 6 and 24 hours, and upgrading at 7 days, culminating at 3.16 ± 1.14 at 28 days. Egr-1 protein expression was detected at 2 hours, with the positive expression rate of 31% ±6%. The level of Egr-1 protein was decreased from 24 hours to 3 days. A peak was reached at 41% ± 8% at 28 days. Immunoreative Egr-1 was located within vascular smooth muscle cells （VSMCs） and monocytes/macrophages in the media at the early phase of day 7 and 14, and in neointimal and medial VSMCs at later phase of day 28. Egr-1 was also present in the endolumial endothelial cells. Conclusion In autogenous vein graft, Egr-1 plays an important role in the proliferation of VSMCs in autogenous vein graft.