中文摘要：

目的：探讨血小板源性生长因子B（PDGF-B）基因转染修饰大鼠骨髓闻克质干细胞（MSC）的可行性。方法：应用FAM标记重组真核表达载体系统（pcDNA3-PDGF-B）。以脂质体法转染原代MSC。观察转染结果、表达情况孕对靶细胞活力的影响。结果：MSC的基因转染成功，持续表达时间超过了8周，没有发现明显的细胞毒作用及对细胞活力的显著影响。结论：采用真柱转染技术可以介导外源基因转染MSC，MSC是一种理想的基因载体细胞。可用于PDGF-B的基因治疗。

英文摘要：

Objective： To study the feasibility of transfecting platelet-derived growth factoroB （PDGF-B） gene to bone marrow mesenehymal stem cells （MSCs）. Methods： Recombinant eukaryofic expression carrier system （ pcDNA3-PDGF-B） was marked with carboxyfluorescein （FAM）. Original MSCs were transfected by using liposome technique. The results of transfection and the activity of the target cell were determined. Results： Bone marrow MSCs were successfully transfected with pcDNA3-PDGF-B. The expression of exogenous gene continued over 8 weeks. No cell toxicity and obvious influence on cell activity was found. Conclusion： MSCs can be efficiently transfected with exogenous gene by using eukaryotic transfection technique. MSCs are ideal cells for cell-mediated transfection and can be used in PDGF-B gene therapy.