中文摘要：

目的：观察艾灸预处理对应激性胃黏膜损伤大鼠胃组织细胞外调节蛋白激酶（extracellularsignal—regulated kinase，ERK）信号转导通路中关键因子——表皮生长因子受体（epidermal growth factor receptor,EGFR）、磷酸化细胞外信号调节激酶（phosphorylation extracellular signal—regulated kinase1／2，p-ERK蛇）与激活蛋白1（activated protein-1，AP．1）蛋白表达的影响，探讨艾灸促进胃黏膜损伤修复的作用机制。方法：将30只Sprague—Dawley（SD）大鼠按照随机数字表随机分为正常组、模型组、艾灸组，每组10只。除正常组外，其余2组大鼠采用束缚冷应激法制作应激性胃黏膜损伤模型。在造模之前，艾灸组大鼠交替灸足三里、中脘或脾俞、胃俞，每日1次，共8d。光镜下观察胃黏膜组织形态学变化，免疫组织化学法检测胃组织p-ERK1/2的表达，蛋白质印迹法检测EGFR与AP-1蛋白的水平。结果：与正常组比较，光镜下模型组大鼠胃黏膜损伤严重，且胃组织EGFR、p-ERK1／2与AP-1蛋白表达升高（P〈0．01，P〈O．05，P〈0．05）；与模型组比较，光镜下艾灸组大鼠胃黏膜损伤较轻，且胃组织EGFR、p-ERK1／2与AP-1蛋白表达升高（均P〈0．01）。结论：艾灸足三里、中脘、脾俞和胃俞能提高应激性胃黏膜损伤大鼠胃组织EGFR、p-ERK1／2、AP-1的表达水平，维持ERK信号转导通路的信息传递功能，促进胃黏膜损伤修复。

英文摘要：

Objective： To observe the effects of moxibustion pretreatment on the protein expressions of epidermal growth factor receptor （EGFR）, phosphorylation extracellular signal-regulated kinase I/2 （p-ERKI/2） and activated protein-1 （AP-2）, the key factors of extracellular signal-regulated kinase signaling transduction pathway in gastric tissue of rats with stress-induced gastric mucosal damage, and to discuss the mechanisms of moxibustion therapy in promoting the restoration of damaged gastric mucosa. Methods： Thirty Sprague-Dawley （SD） rats were randomly divided into a normal group, a model group, and a moxibustion group using the random digits table, 10 in each group. Except the rats in the normal group, rats in the other two groups were used to make stress-induced gastric mucosal damage model using restraint and cold stress. Before modeling, rats in the moxibustion group were alternately treated with moxibustion a/t Zusanli （ST 36） and Zhongwan （CV 12）, or Pishu （BL 20） and Weishu （BL 22）, once a day, for a total of 8 d. Histolo~cal changes of gastric mucosa were observed under the light microscopy, the expression of gastric tissue p-ERKI/2 was detected by immunohistochemistry assay, and the protein levels of EGFR and AP-I were measured by Western blots. Results： Compared with rats in the normal group, gastric mucosal damage was more serious, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the model group （P〈0.01, P〈O.05, P〈0.05）. Compared with rats in the model group, gastric mucosal damage was milder, and protein expressions of gastric tissue EGFR, p-ERK1/2 and AP-1 increased in the moxibustion group （all P〈0.01）. Conclusion： Moxibustion at Zusanli （ST 36）, Zhongwan （CV 12）, Pishu （BL 20） and Weishu （BL 21） could increase EGFR, p-ERK1/2 and AP-1 expression levels in gastric tissue of stress-induced gastric mucosal damage rats, maintain the information transfer function of ERK signaling transduction pathway, and p