中文摘要：

目的：基于核磁共振氢谱代谢组学技术（^1H nuclear magnetic resonance,^1H NMR）探讨艾灸对正常人体血清代谢的影响,并寻找差异性代谢物,从整体代谢的角度阐述艾灸对健康人体的影响。方法：将60例在校健康青年男性采用随机数字表随机分成艾灸组和对照组,每组30例。艾灸组予温和灸右侧足三里,每天1次,每次15 min,连续治疗10 d;对照组不予任何干预。干预结束后艾灸组剩余28例,对照组剩余23例。在干预第1d、第5d和第10 d,采集两组受试者的血清样品,运用1H NMR技术获取代谢图谱。结果：艾灸组干预前后血清^1H NMR有明显差异,对照组干预前后1H NMR无明显差异。艾灸组代谢物的变化主要是低密度脂蛋白（low density lipoprotein,LDL）/极低密度脂蛋白（very low density lipoprotein,VLDL）、缬氨酸、异亮氨酸、亮氨酸、乳酸、谷氨酰胺、柠檬酸、多不饱和脂肪酸、肌酸、甘氨酸、甘油、葡萄糖、酪氨酸、组氨酸、甲酸、丙氨酸、赖氨酸、乙酸、谷氨酸。结论：艾灸能够引起正常人体血清代谢模式变化,通过影响支链氨基酸、多不饱和脂肪酸等代谢物浓度加强机体的氨基酸、脂肪酸代谢。

英文摘要：

Objective： To investigate the effects of moxibustion on the serum metabolism in healthy human body based on the 1H nuclear magnetic resonance （1H NMR） metabolomics technology, and to find the differences in metabolites, as well as to elucidate the effects of moxibustion on healthy human body from the viewpoint of global metabolism. Methods： Sixty subjects of healthy young men from the enrolled students were randomly divided into a moxibustion group and a control group using random number table, with 30 cases in each group. Subjects in the moxibustion group accepted mild moxibustion on the right Zusanli （ST 36）, once a day, 15 min for each time, and continuous treatment for 10 d; those in the control group did not receive any intervention. There were 28 cases in the moxibustion group and 23 cases in the control group after interventions. On the 2st day, 5th day and lOth day of the intervention, serum samples were collected from subjects of the two groups, and metabolic spectra were obtained by the 1H NMR technology. Results： Before and after the intervention, serum 1H NMR of the moxibustion group was significantly different, while the difference was insignificant in the control group. Metabolite changes in the moxibustion group were mainly in low density lipoprotein （LDL）/very low density lipoprotein （VLDL）, valine, isoleucine, leucine, lactic acid, glutamine, citric acid, polyunsaturated fatty acids, creatine, glycine, glycerol, glucose, tyrosine, histidine, formic acid, alanine, lysine, acetic acid, and glutamic acid. Conclusion： Moxibustion can cause changes of serum metabolic patterns in healthy human by influencing the concentrations of branched-chain amino acids, polyunsaturated fatty acids, and other metabolites to strengthen body＇s metabolisms of amino acids and fatty acid.