中文摘要：

目的：观察隔药饼灸对结肠5-HT及中枢c-fos表达的影响,探讨隔药饼灸对功能性胃肠病（FGIDs）肝郁脾虚证大鼠治疗作用的可能机制。方法：将48只SD大鼠按随机数字表法随机分为4组,即：空白组、模型组、隔药饼灸组和逍遥散组,每组12只。空白组正常饲养,不做任何处理;余3组均采用复合病因造模法（慢性束缚应激＋过度疲劳＋饮食失节＋夹尾＋摇晃）连续21d造模后,模型组行捆绑束缚30min,并灌服0.9%氯化钠溶液,灌胃容积均为1mL/100g体质量（下同）;隔药饼灸组行隔药饼灸30min,并灌服0.9%氯化钠溶液;逍遥散组行捆绑束缚30min,灌服逍遥散,连续干预14d。实验结束后采用高效液相检测结肠5-HT表达,荧光定量PCR法检测海马、杏仁核c-fos表达。结果：与空白组相比,模型组大鼠结肠组织中的5-HT的表达明显增加（P〈0.01）;与模型组相比,逍遥散组和隔药饼灸组结肠组织中5-HT表达下降（P〈0.01,P〈0.05）;且隔药饼灸组优于逍遥散组（P〈0.05）。与空白组相比,模型组c-fos表达均增多（P〈0.05）;与模型组相比,隔药饼灸组和逍遥散组c-fos表达减少（P〈0.05）;隔药饼灸组优于逍遥散组（P〈0.05）。结论：隔药饼灸能下调结肠组织中5-HT表达来改善胃肠吸收和感觉异常,结肠5-HT及中枢海马、杏仁核c-fos异常表达提示FGIDs发病机制不仅与内分泌免疫系统方面有关,同时神经系统也参与该病发生,提示脑肠轴-中枢途径可能是隔药饼灸治疗FGIDs关键所在。

英文摘要：

Objective： To observe the influence of cake-separated moxibustion on the expression of 5-HT and central c-los in rats with functional gastrointestinal disorders （FGIDs）, and to investigate the possible mechanism and therapeutic effect of cake-separated moxibustion in rats with FGIDs of liver-depression and spleen-deficiency syndrome. Methods： 48 SD rats were divided into 4 groups according to the table of random number： a blank group （A）, a model group （B）, cake-separated moxibustion group （C）, Xiaoyao Powder group （D）, 12 in each group. Rats in group A were normal feeding without any treatment. The rest three groups were made with complex modeling methods for 21 days （chronic restraint stress ＋ excessive fatigue ＋ irregular food ＋ tail clamp ＋ shake）. Group B was bounded for 30min and given normal saline by gavage, and the gavage volume was 1mL/100g （the same below）. Group C was treated with cake-separated moxibustion for 30min, and given normal saline by gavage. Group D was bounded for 30min, and given Xiaoyao Powder by gavage for 14 days. After the experiment finished, the expression of 5-HT in colon tissues was detected by high performance liquid chromatography （HPLC）, and the expression of c-fos in hippocampus and amygdala were detected by fluorescence quantitative PCR. Results： Compared with the blank group, the expression of 5-HT in colon tissues of rats in the model group was statistically significantly different （P〈0.01）. Compared with the model group, the expression of 5-HT in colon tissue of rats decreased in Xiaoyao Powder group and cake-separated moxibustion group, and the differences were statistically significant （P〈0.05, P〈0.01）. Compared with Xiaoyao Powder group, the difference in cake-separated moxibustion group was statistically significance （P〈0.05）. Compared with the blank group, the expression of c-fos in hippocampus and amygdala was statistically significant in the model group （P〈0.05）. Compared with the model group,