中文摘要：

目的：观察鼻咽癌密切相关基因BRD7与接头相关蛋白复合物3（AP3）δ亚单位的相互作用及功能。方法：首先构建AP3δ基因真核表达载体pCMV-HA-AP3δ和pEGFP-C2-AP3δ,然后采用GFP介导的细胞荧光实验考察非洲绿猴肾COS7细胞中AP3δ的亚细胞定位;通过细胞荧光共定位和免疫共沉淀实验分析BRD7蛋白与AP3δ蛋白的相互作用;最后采用荧光素酶实验观察BRD7与AP3δ对COS7细胞E2F3和CyclinD1活性的影响,半定量逆转录PCR观察转染pcDNA3.1-BRD7的HNE1细胞AP3δmRNA的表达,以转染空白载体的HNE1细胞为对照。结果：成功构建了pCMV-HA-AP3δ和pEGFP-C2-AP3δ;AP3δ蛋白主要定位于胞质;AP3δ蛋白与BRD7蛋白存在相互作用。AP3δ基因可协同BRD7下调细胞E2F3和CyclinD1启动子活性。在BRD7稳定转染的HNE1细胞中,AP3δmRNA的表达较转染空白载体组明显上调。结论：AP3δ蛋白与BRD7蛋白存在相互作用,在功能上相互促进。

英文摘要：

Aim： To investigate the interaction and function of BRD7 and adaptor-related protein complex 3δ （AP3δ）. Methods：Firstly, pCMV-HA-AP＇3δ and pEGFP-C2-AP3δ were constructed. Secondly, the subcellular localiza- tion of AP3δ was detected in COS7 cells by direct GFP fluorescence, and the interaction between BRD7 and AP3δ protein was further confirmed by coimmunoprecipitation and immunocolocalizafion. Lastly, luciferase assay was used to observe the activity of E2F3 and CyelinD1 in COS7 cells after transfected by BRD7 and AP3δ alone or combined, and RT-PCR was used to observe the changes of AP3δ mRNA in HNE1 cells after transfeeted by peDNA3.1-BRD7. Results： pCMV-HA- AP3δ and pEGFP-C2-AP3δ were constructed successfully. AP3δ was mainly located in nuclei of COS7 cells. Compared with BRD7 transfeetion alone,the combination of BRD7 and AP3δ had interactive effects on down-regulating the activity of E2F3 and CyclinD1. Compared with the HNE1 cells transfeeted by blank plasmid, the expression level of AP3B mRNA in- creased in the HNEI cells transfected by peDNA3. I-BRD7. Conclusion ： The function of BRD7 and AP3δ protein may be promoted mutually with the interaction between BRD7 and AP3δ protein.