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中文摘要：
BRD7基因是采用cDNA代表性差异分析法克隆得到的一个新的Bromodomain基因（GenBanl（登录号AF152604）．它在鼻咽癌细胞和组织中表达明显下调，过表达BRD7基因能部分逆转鼻咽癌细胞的恶性表型．为了揭示BRD7基因在鼻咽癌细胞和组织中表达下调的分子机制，利用生物信息学技术已预测出其启动子区．荧光素酶活性检测结果表明该区域具有强启动子活性；转录因子Sp1特异性地结合于BRD7该启动子区；Sp1特异性阻断剂mithramycin A能明显地抑制BRD7启动子的活性和BRD7基因的表达．
英文摘要：
BRD7, a novel bromodomain gene, has been cloned by cDNA RDA （cDNA Representational Difference Analysis）. The GenBank accession number is AF152604. Previous studies showed that BRD7 gene was down-regulated in nasopharyngeal carcinoma （NPC） cells and tissues. Over expression of BRD7 in NPC cells can inhibit cell proliferation and cell cycle progression from G1 to S phase, and can partly inhibit the aberrant growth of NPC cells. In order to explore the molecular mechanisms that involved in the down-regulation of BRD7 gene in NPC cells, bioinformatic approaches were adopted and a putative promoter region was found. The luciferase expression vectors containing BRD7 promoter region were further constructed . Transient transfection results suggested that the analyzed sequence contained BRD7 promoter. Transcriptional factor Spl is responsive to this region. Inhibition of the Spl binding to BRD7 promoter by mithramycin A significantly reduced the promoter activity and the endogenous expression of BRD7 in mRNA level.
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